Base composition(content of A,T,G,C): Analysis
CpG dinucleotides: Analysis
Analyzed base type(single base or their composition):
Window of local GC content plot(bp):
Summary of parameters:
Min length of ORF(AAs): Max length of ORF(AAs):(0:any length)
+1 Open reading frame: first base of sense strand as initiation site of ORF
+2 Open reading frame: second base of sense strand as initiation site of ORF
+3 Open reading frame: third base of sense strand as initiation site of ORF
-1 Open reading frame: first base of antisense strand as initiation site of ORF
-2 Open reading frame: second base of antisense strand as initiation site of ORF
-3 Open reading frame: third base of antisense strand as initiation site of ORF
Alternative Reading Frame(ARF): Sense Anti-senseConfidence(percent):(0-1.0)
Cis-type: Mammalian Bacteria Insect Yeast Plant other
Codon analysis:
Codon usage table: Human Ecoli Insect Yeast Mouse Rat Maize Tobacco
Others:Codon usage tables
My codon table::
Min length of repeat(bp):
Computing Tm of repeat using the following parameters:
Thermodynamic parameters: Santalucia Breslauter Salt correction formula: Owczarzy Schildkraut Santalucia
Concentration of monovalent cations(mM) Concentration of dNTP(mM)
Concentration of divalent cations(mM) Annealing oligo concentration(nM)
Analyze cis-acting elements:
My motifs:(format:(TTTTTTT):(AT{3,10}A))
My restriction sites:(format:NdeI(CATATG):(CATATG))