M13 fwd M13 fwd(379-395) M13 f... M13 fwd(379-395) M13 f... M13 fwd(379-395) MCS MCS(396-476) MCS MCS(396-476) M13 rev M13 rev(489-505) M13 r... M13 rev(489-505) M13 r... M13 rev(489-505) lac operator lac operator(513-529) lac o... lac operator(513-529) lac o... lac operator(513-529) lac promoter lac promoter(537-567) lac p... lac promoter(537-567) lac p... lac promoter(537-567) ori ori(891-1479) ori ori(891-1479) AmpR AmpR(1650-2510) AmpR AmpR(1650-2510) AmpR promoter AmpR promoter(2511-2615) AmpR ... AmpR promoter(2511-2615) ...AmpR AmpR promoter(2511-2615) M13 rev M13 rev(491-507) M13 r... M13 rev(491-507) M13 r... M13 rev(491-507) M13 fwd M13 fwd(381-397) M13 f... M13 fwd(381-397) M13 f... M13 fwd(381-397) lac operator lac operator(515-531) lac o... lac operator(515-531) lac o... lac operator(515-531) CAP binding site CAP binding site(584-605) CAP b... CAP binding site(584-605) CAP b... CAP binding site(584-605) lac promoter lac promoter(539-569) lac p... lac promoter(539-569) lac p... lac promoter(539-569) AmpR promoter AmpR promoter(2513-2617) AmpR ... AmpR promoter(2513-2617) ...AmpR AmpR promoter(2513-2617)