lacZα lacZα(16-363) lacZα lacZα(16-363) M13 fwd M13 fwd(249-265) M13 f... M13 fwd(249-265) M13 f... M13 fwd(249-265) M13 rev M13 rev(359-375) M13 r... M13 rev(359-375) M13 r... M13 rev(359-375) lac operator lac operator(383-399) lac o... lac operator(383-399) lac o... lac operator(383-399) lac promoter lac promoter(407-437) lac p... lac promoter(407-437) lac p... lac promoter(407-437) CAP binding site CAP binding site(452-473) CAP b... CAP binding site(452-473) CAP b... CAP binding site(452-473) rop rop(1161-1352) rop rop(1161-1352) bom bom(1454-1594) bom bom(1454-1594) pBR322 Ori pBR322 Ori(1724-2391) pBR32... pBR322 Ori(1724-2391) ori ori(1780-2368) ori ori(1780-2368) kan kan(2539-3354) kan kan(2539-3354) AmpR promoter AmpR promoter(3355-3459) AmpR ... AmpR promoter(3355-3459) ...AmpR AmpR promoter(3355-3459) M13 rev M13 rev(361-377) M13 r... M13 rev(361-377) M13 r... M13 rev(361-377) M13 fwd M13 fwd(251-267) M13 f... M13 fwd(251-267) M13 f... M13 fwd(251-267) lac operator lac operator(385-401) lac o... lac operator(385-401) lac o... lac operator(385-401) CAP binding site CAP binding site(454-475) CAP b... CAP binding site(454-475) CAP b... CAP binding site(454-475) lac promoter lac promoter(409-439) lac p... lac promoter(409-439) lac p... lac promoter(409-439) AmpR promoter AmpR promoter(3357-3461) AmpR ... AmpR promoter(3357-3461) ...AmpR AmpR promoter(3357-3461) bom bom(1456-1596) bom bom(1456-1596) rop rop(1163-1354) rop rop(1163-1354) ori ori(1782-2370) ori ori(1782-2370)