ori ori(795-1383) ori ori(795-1383) AmpR AmpR(1554-2414) AmpR AmpR(1554-2414) AmpR promoter AmpR promoter(2415-2519) AmpR ... AmpR promoter(2415-2519) AmpR ... AmpR promoter(2415-2519) copia promoter copia promoter(4001-4280) copia... copia promoter(4001-4280) copia... copia promoter(4001-4280) HygR HygR(4326-5351) HygR HygR(4326-5351) small t intron small t intron(5714-5779) small... small t intron(5714-5779) small... small t intron(5714-5779) SV40 NLS SV40 NLS(5909-5929) SV40 ... SV40 NLS(5909-5929) SV40 ... SV40 NLS(5909-5929) SV40 poly(A) signal SV40 poly(A) signal(6354-6488) SV40 ... SV40 poly(A) signal(6354-6488) SV40 ... SV40 poly(A) signal(6354-6488) MT promoter MT promoter(6489-6915) MT pr... MT promoter(6489-6915) MT pr... MT promoter(6489-6915) KS primer KS primer(6924-6940) KS pr... KS primer(6924-6940) ...KS pr KS primer(6924-6940) attB1 attB1(6955-6979) attB1 attB1(6955-6979) attB1 attB1(6955-6979) Kozak sequence Kozak sequence(6994-7003) Kozak... Kozak sequence(6994-7003) ...Kozak Kozak sequence(6994-7003) 3xFLAG 3xFLAG(7003-7068) 3xFLA... 3xFLAG(7003-7068) ...3xFLA 3xFLAG(7003-7068) SV40 NLS SV40 NLS(7075-7095) SV40 ... SV40 NLS(7075-7095) ...SV40 SV40 NLS(7075-7095) Cas9 Cas9(7120-11220) Cas9 Cas9(7120-11220) nucleoplasmin NLS nucleoplasmin NLS(11221-11268) nucle... nucleoplasmin NLS(11221-11268) ...nucle nucleoplasmin NLS(11221-11268) bGH poly(A) signal bGH poly(A) signal(11308-11515) bGH p... bGH poly(A) signal(11308-11515) ...bGH p bGH poly(A) signal(11308-11515) attB2 attB2(11553-11577) attB2 attB2(11553-11577) attB2 attB2(11553-11577) APEX2 APEX2(11600-12346) APEX2 APEX2(11600-12346) Myc Myc(12365-12394) Myc Myc(12365-12394) Myc Myc(12365-12394) Myc Myc(12398-12427) Myc Myc(12398-12427) Myc Myc(12398-12427) Myc Myc(12431-12460) Myc Myc(12431-12460) Myc Myc(12431-12460) KS primer KS primer(6926-6942) KS pr... KS primer(6926-6942) ...KS pr KS primer(6926-6942) attB2 attB2(11558-11578) attB2 attB2(11558-11578) attB2 attB2(11558-11578) attB1 attB1(6958-6978) attB1 attB1(6958-6978) attB1 attB1(6958-6978) SV40 NLS SV40 NLS(5911-5931) SV40 ... SV40 NLS(5911-5931) SV40 ... SV40 NLS(5911-5931) small t intron small t intron(5716-5781) small... small t intron(5716-5781) small... small t intron(5716-5781) AmpR promoter AmpR promoter(2417-2521) AmpR ... AmpR promoter(2417-2521) AmpR ... AmpR promoter(2417-2521) SV40 poly(A) signal SV40 poly(A) signal(6356-6490) SV40 ... SV40 poly(A) signal(6356-6490) SV40 ... SV40 poly(A) signal(6356-6490) ori ori(797-1385) ori ori(797-1385) AmpR AmpR(1556-2416) AmpR AmpR(1556-2416) HygR HygR(4328-5353) HygR HygR(4328-5353)