J23119(SpeI) promoter J23119(SpeI) promoter(5-39) J2311... J23119(SpeI) promoter(5-39) J2311... J23119(SpeI) promoter(5-39) gRNA scaffold gRNA scaffold(60-135) gRNA ... gRNA scaffold(60-135) gRNA ... gRNA scaffold(60-135) Myc Myc(156-185) Myc Myc(156-185) Myc Myc(156-185) 6xHis 6xHis(201-218) 6xHis 6xHis(201-218) 6xHis 6xHis(201-218) rrnB T1 terminator rrnB T1 terminator(444-490) rrnB ... rrnB T1 terminator(444-490) rrnB ... rrnB T1 terminator(444-490) ori ori(714-1302) ori ori(714-1302) AmpR AmpR(1473-2333) AmpR AmpR(1473-2333) AmpR promoter AmpR promoter(2334-2438) AmpR ... AmpR promoter(2334-2438) ...AmpR AmpR promoter(2334-2438) bacterial terminator bacterial terminator(2528-2571) bacte... bacterial terminator(2528-2571) ...bacte bacterial terminator(2528-2571) bacterial terminator bacterial terminator(2530-2573) bacte... bacterial terminator(2530-2573) ...bacte bacterial terminator(2530-2573) gRNA scaffold gRNA scaffold(62-137) gRNA ... gRNA scaffold(62-137) gRNA ... gRNA scaffold(62-137) AmpR promoter AmpR promoter(2336-2440) AmpR ... AmpR promoter(2336-2440) ...AmpR AmpR promoter(2336-2440)