J23119(SpeI) promoter J23119(SpeI) promoter(192-226) J2311... J23119(SpeI) promoter(192-226) J2311... J23119(SpeI) promoter(192-226) gRNA scaffold gRNA scaffold(247-322) gRNA ... gRNA scaffold(247-322) gRNA ... gRNA scaffold(247-322) SmR SmR(508-1299) SmR SmR(508-1299) ori ori(1473-2061) ori ori(1473-2061) gRNA scaffold gRNA scaffold(249-324) gRNA ... gRNA scaffold(249-324) gRNA ... gRNA scaffold(249-324) ori ori(1475-2063) ori ori(1475-2063) SmR SmR(510-1301) SmR SmR(510-1301)