TetR TetR(7-633) TetR TetR(7-633) tetR/tetA promoters tetR/tetA promoters(649-704) tetR/... tetR/tetA promoters(649-704) tetR/... tetR/tetA promoters(649-704) tet operator tet operator(685-703) tet o... tet operator(685-703) tet o... tet operator(685-703) RBS RBS(722-733) RBS RBS(722-733) RBS RBS(722-733) Cas9 Cas9(740-4843) Cas9 Cas9(740-4843) Cas9 Cas9(740-4846) Cas9 Cas9(740-4846) rrnB T1 terminator rrnB T1 terminator(4870-4941) rrnB ... rrnB T1 terminator(4870-4941) ...rrnB rrnB T1 terminator(4870-4941) T7Te terminator T7Te terminator(4957-4984) T7Te ... T7Te terminator(4957-4984) ...T7Te T7Te terminator(4957-4984) ori ori(5151-5739) ori ori(5151-5739) lambda t0 terminator lambda t0 terminator(5827-5921) lambd... lambda t0 terminator(5827-5921) ...lambd lambda t0 terminator(5827-5921) AmpR AmpR(5951-6811) AmpR AmpR(5951-6811) AmpR promoter AmpR promoter(6812-6916) AmpR ... AmpR promoter(6812-6916) ...AmpR AmpR promoter(6812-6916) tet operator tet operator(687-705) tet o... tet operator(687-705) tet o... tet operator(687-705) T7Te terminator T7Te terminator(4959-4986) T7Te ... T7Te terminator(4959-4986) ...T7Te T7Te terminator(4959-4986) lambda t0 terminator lambda t0 terminator(5829-5923) lambd... lambda t0 terminator(5829-5923) ...lambd lambda t0 terminator(5829-5923) AmpR promoter AmpR promoter(6814-6918) AmpR ... AmpR promoter(6814-6918) ...AmpR AmpR promoter(6814-6918) ori ori(5153-5741) ori ori(5153-5741) TetR TetR(9-632) TetR TetR(9-632)