5' LTR 5' LTR(217-397) 5' LT... 5' LTR(217-397) 5' LT... 5' LTR(217-397) HIV-1 ψ HIV-1 ψ(444-569) HIV-1... HIV-1 ψ(444-569) HIV-1... HIV-1 ψ(444-569) HIV-1 RRE HIV-1 RRE(1062-1295) HIV-1... HIV-1 RRE(1062-1295) HIV-1... HIV-1 RRE(1062-1295) HIV-1 cPPT HIV-1 cPPT(1822-1939) HIV-1... HIV-1 cPPT(1822-1939) HIV-1... HIV-1 cPPT(1822-1939) FLAG FLAG(8645-8668) FLAG FLAG(8645-8668) FLAG FLAG(8645-8668) PuroR PuroR(8735-9331) PuroR PuroR(8735-9331) WPRE WPRE(9347-9935) WPRE WPRE(9347-9935) Factor Xa site Factor Xa site(9818-9829) Facto... Factor Xa site(9818-9829) ...Facto Factor Xa site(9818-9829) 3'LTR (ΔU3) 3'LTR (ΔU3)(10007-10240) 3'LTR... 3'LTR (ΔU3)(10007-10240) ...3'LTR 3'LTR (ΔU3)(10007-10240) 5' LTR 5' LTR(10060-10240) 5' LT... 5' LTR(10060-10240) ...5' LT 5' LTR(10060-10240) bGH poly(A) signal bGH poly(A) signal(10272-10496) bGH p... bGH poly(A) signal(10272-10496) ...bGH p bGH poly(A) signal(10272-10496) f1 ori f1 ori(10542-10970) f1 or... f1 ori(10542-10970) ...f1 or f1 ori(10542-10970) SV40 promoter SV40 promoter(10984-11313) SV40 ... SV40 promoter(10984-11313) ...SV40 SV40 promoter(10984-11313) SV40 ori SV40 ori(11164-11299) SV40 ... SV40 ori(11164-11299) ...SV40 SV40 ori(11164-11299) EM7 promoter EM7 promoter(11361-11408) EM7 p... EM7 promoter(11361-11408) ...EM7 p EM7 promoter(11361-11408) BleoR BleoR(11427-11801) BleoR BleoR(11427-11801) BleoR BleoR(11427-11801) SV40 poly(A) signal SV40 poly(A) signal(11931-12052) SV40 ... SV40 poly(A) signal(11931-12052) ...SV40 SV40 poly(A) signal(11931-12052) M13 rev M13 rev(12101-12117) M13 r... M13 rev(12101-12117) ...M13 r M13 rev(12101-12117) lac operator lac operator(12125-12141) lac o... lac operator(12125-12141) ...lac o lac operator(12125-12141) lac promoter lac promoter(12149-12179) lac p... lac promoter(12149-12179) ...lac p lac promoter(12149-12179) ori ori(12503-13091) ori ori(12503-13091) AmpR AmpR(13262-14122) AmpR AmpR(13262-14122) AmpR promoter AmpR promoter(14123-14227) AmpR ... AmpR promoter(14123-14227) ...AmpR AmpR promoter(14123-14227) CMV enhancer CMV enhancer(14493-14872) CMV e... CMV enhancer(14493-14872) ...CMV e CMV enhancer(14493-14872) Factor Xa site Factor Xa site(9820-9831) Facto... Factor Xa site(9820-9831) ...Facto Factor Xa site(9820-9831) M13 rev M13 rev(12103-12119) M13 r... M13 rev(12103-12119) ...M13 r M13 rev(12103-12119) lac operator lac operator(12127-12143) lac o... lac operator(12127-12143) ...lac o lac operator(12127-12143) CAP binding site CAP binding site(12196-12217) CAP b... CAP binding site(12196-12217) ...CAP b CAP binding site(12196-12217) lac promoter lac promoter(12151-12181) lac p... lac promoter(12151-12181) ...lac p lac promoter(12151-12181) EM7 promoter EM7 promoter(11363-11410) EM7 p... EM7 promoter(11363-11410) ...EM7 p EM7 promoter(11363-11410) gRNA scaffold gRNA scaffold(4122-4197) gRNA ... gRNA scaffold(4122-4197) gRNA ... gRNA scaffold(4122-4197) AmpR promoter AmpR promoter(14125-14229) AmpR ... AmpR promoter(14125-14229) ...AmpR AmpR promoter(14125-14229) cPPT/CTS cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) HIV-1 Ψ HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) bGH poly(A) signal bGH poly(A) signal(10274-10498) bGH p... bGH poly(A) signal(10274-10498) ...bGH p bGH poly(A) signal(10274-10498) RRE RRE(1064-1297) RRE RRE(1064-1297) RRE RRE(1064-1297) 3' LTR (ΔU3) 3' LTR (ΔU3)(10009-10242) 3' LT... 3' LTR (ΔU3)(10009-10242) ...3' LT 3' LTR (ΔU3)(10009-10242) U6 promoter U6 promoter(1992-2232) U6 pr... U6 promoter(1992-2232) U6 pr... U6 promoter(1992-2232) BleoR BleoR(11429-11803) BleoR BleoR(11429-11803) BleoR BleoR(11429-11803) CMV enhancer CMV enhancer(14495-1) CMV e... CMV enhancer(14495-1) ...CMV e CMV enhancer(14495-1) WPRE WPRE(9349-9937) WPRE WPRE(9349-9937)