SV40 ori SV40 ori(55-190) SV40 ... SV40 ori(55-190) SV40 ... SV40 ori(55-190) SV40 promoter SV40 promoter(41-370) SV40 ... SV40 promoter(41-370) SV40 ... SV40 promoter(41-370) f1 ori f1 ori(384-812) f1 or... f1 ori(384-812) f1 or... f1 ori(384-812) bGH poly(A) signal bGH poly(A) signal(858-1082) bGH p... bGH poly(A) signal(858-1082) bGH p... bGH poly(A) signal(858-1082) 3' LTR (ΔU3) 3' LTR (ΔU3)(1114-1347) 3' LT... 3' LTR (ΔU3)(1114-1347) 3' LT... 3' LTR (ΔU3)(1114-1347) Factor Xa site Factor Xa site(1525-1536) Facto... Factor Xa site(1525-1536) Facto... Factor Xa site(1525-1536) WPRE WPRE(1419-2007) WPRE WPRE(1419-2007) EGFP EGFP(2026-2742) EGFP EGFP(2026-2742) P2A P2A(2743-2799) P2A P2A(2743-2799) P2A P2A(2743-2799) FLAG FLAG(2809-2832) FLAG FLAG(2809-2832) FLAG FLAG(2809-2832) nucleoplasmin NLS nucleoplasmin NLS(2833-2880) nucle... nucleoplasmin NLS(2833-2880) nucle... nucleoplasmin NLS(2833-2880) Cas9 Cas9(2881-6984) Cas9 Cas9(2881-6984) Kozak sequence Kozak sequence(6981-6990) Kozak... Kozak sequence(6981-6990) ...Kozak Kozak sequence(6981-6990) EF-1α core promoter EF-1α core promoter(7009-7220) EF-1α... EF-1α core promoter(7009-7220) ...EF-1α EF-1α core promoter(7009-7220) gRNA scaffold gRNA scaffold(7282-7357) gRNA ... gRNA scaffold(7282-7357) ...gRNA gRNA scaffold(7282-7357) U6 promoter U6 promoter(7382-7622) U6 pr... U6 promoter(7382-7622) ...U6 pr U6 promoter(7382-7622) cPPT/CTS cPPT/CTS(7673-7790) cPPT/... cPPT/CTS(7673-7790) ...cPPT/ cPPT/CTS(7673-7790) gp41 peptide gp41 peptide(8088-8132) gp41 ... gp41 peptide(8088-8132) ...gp41 gp41 peptide(8088-8132) RRE RRE(8317-8550) RRE RRE(8317-8550) RRE RRE(8317-8550) HIV-1 Ψ HIV-1 Ψ(9043-9168) HIV-1... HIV-1 Ψ(9043-9168) ...HIV-1 HIV-1 Ψ(9043-9168) 5' LTR (truncated) 5' LTR (truncated)(9215-9395) 5' LT... 5' LTR (truncated)(9215-9395) ...5' LT 5' LTR (truncated)(9215-9395) CMV promoter CMV promoter(9413-9611) CMV p... CMV promoter(9413-9611) ...CMV p CMV promoter(9413-9611) CMV enhancer CMV enhancer(9613-9992) CMV e... CMV enhancer(9613-9992) ...CMV e CMV enhancer(9613-9992) AmpR promoter AmpR promoter(10258-10362) AmpR ... AmpR promoter(10258-10362) ...AmpR AmpR promoter(10258-10362) AmpR AmpR(10363-11223) AmpR AmpR(10363-11223) ori ori(11394-11982) ori ori(11394-11982) CAP binding site CAP binding site(12270-12291) CAP b... CAP binding site(12270-12291) ...CAP b CAP binding site(12270-12291) lac promoter lac promoter(12306-12336) lac p... lac promoter(12306-12336) ...lac p lac promoter(12306-12336) lac operator lac operator(12344-12360) lac o... lac operator(12344-12360) ...lac o lac operator(12344-12360) M13 rev M13 rev(12368-12384) M13 r... M13 rev(12368-12384) ...M13 r M13 rev(12368-12384) SV40 poly(A) signal SV40 poly(A) signal(12433-12554) SV40 ... SV40 poly(A) signal(12433-12554) ...SV40 SV40 poly(A) signal(12433-12554) BleoR BleoR(12684-13058) BleoR BleoR(12684-13058) BleoR BleoR(12684-13058) EM7 promoter EM7 promoter(13077-13124) EM7 p... EM7 promoter(13077-13124) ...EM7 p EM7 promoter(13077-13124) Factor Xa site Factor Xa site(1527-1538) Facto... Factor Xa site(1527-1538) Facto... Factor Xa site(1527-1538) M13 rev M13 rev(12370-12386) M13 r... M13 rev(12370-12386) ...M13 r M13 rev(12370-12386) lac operator lac operator(12346-12362) lac o... lac operator(12346-12362) ...lac o lac operator(12346-12362) CAP binding site CAP binding site(12272-12293) CAP b... CAP binding site(12272-12293) ...CAP b CAP binding site(12272-12293) lac promoter lac promoter(12308-12338) lac p... lac promoter(12308-12338) ...lac p lac promoter(12308-12338) EM7 promoter EM7 promoter(13079-13126) EM7 p... EM7 promoter(13079-13126) ...EM7 p EM7 promoter(13079-13126) gRNA scaffold gRNA scaffold(7284-7359) gRNA ... gRNA scaffold(7284-7359) ...gRNA gRNA scaffold(7284-7359) AmpR promoter AmpR promoter(10260-10364) AmpR ... AmpR promoter(10260-10364) ...AmpR AmpR promoter(10260-10364) cPPT/CTS cPPT/CTS(7675-7792) cPPT/... cPPT/CTS(7675-7792) ...cPPT/ cPPT/CTS(7675-7792) HIV-1 Ψ HIV-1 Ψ(9045-9170) HIV-1... HIV-1 Ψ(9045-9170) ...HIV-1 HIV-1 Ψ(9045-9170) bGH poly(A) signal bGH poly(A) signal(860-1084) bGH p... bGH poly(A) signal(860-1084) bGH p... bGH poly(A) signal(860-1084) RRE RRE(8319-8552) RRE RRE(8319-8552) RRE RRE(8319-8552) 3' LTR (ΔU3) 3' LTR (ΔU3)(1116-1349) 3' LT... 3' LTR (ΔU3)(1116-1349) 3' LT... 3' LTR (ΔU3)(1116-1349) U6 promoter U6 promoter(7384-7624) U6 pr... U6 promoter(7384-7624) ...U6 pr U6 promoter(7384-7624) BleoR BleoR(12686-13060) BleoR BleoR(12686-13060) BleoR BleoR(12686-13060) CMV enhancer CMV enhancer(9615-9994) CMV e... CMV enhancer(9615-9994) ...CMV e CMV enhancer(9615-9994) WPRE WPRE(1421-2009) WPRE WPRE(1421-2009)