cPPT/CTS cPPT/CTS(41-158) cPPT/... cPPT/CTS(41-158) cPPT/... cPPT/CTS(41-158) tet operator tet operator(220-238) tet o... tet operator(220-238) tet o... tet operator(220-238) tet operator tet operator(256-274) tet o... tet operator(256-274) tet o... tet operator(256-274) tet operator tet operator(291-309) tet o... tet operator(291-309) tet o... tet operator(291-309) tet operator tet operator(327-345) tet o... tet operator(327-345) ...tet o tet operator(327-345) tight TRE promoter tight TRE promoter(212-526) tight... tight TRE promoter(212-526) tight... tight TRE promoter(212-526) tet operator tet operator(363-381) tet o... tet operator(363-381) ...tet o tet operator(363-381) tet operator tet operator(398-416) tet o... tet operator(398-416) ...tet o tet operator(398-416) tet operator tet operator(434-452) tet o... tet operator(434-452) ...tet o tet operator(434-452) Kozak sequence Kozak sequence(543-552) Kozak... Kozak sequence(543-552) ...Kozak Kozak sequence(543-552) 3xFLAG 3xFLAG(552-617) 3xFLA... 3xFLAG(552-617) ...3xFLA 3xFLAG(552-617) SV40 NLS SV40 NLS(624-644) SV40 ... SV40 NLS(624-644) ...SV40 SV40 NLS(624-644) Cas9 Cas9(669-4769) Cas9 Cas9(669-4769) nucleoplasmin NLS nucleoplasmin NLS(4770-4817) nucle... nucleoplasmin NLS(4770-4817) nucle... nucleoplasmin NLS(4770-4817) hPGK promoter hPGK promoter(4827-5337) hPGK ... hPGK promoter(4827-5337) hPGK ... hPGK promoter(4827-5337) BSD BSD(5347-5742) BSD BSD(5347-5742) T2A T2A(5743-5796) T2A T2A(5743-5796) T2A T2A(5743-5796) rtTA-Advanced rtTA-Advanced(5797-6543) rtTA-... rtTA-Advanced(5797-6543) Factor Xa site Factor Xa site(7001-7012) Facto... Factor Xa site(7001-7012) ...Facto Factor Xa site(7001-7012) 3' LTR (ΔU3) 3' LTR (ΔU3)(7217-7450) 3' LT... 3' LTR (ΔU3)(7217-7450) ...3' LT 3' LTR (ΔU3)(7217-7450) SV40 poly(A) signal SV40 poly(A) signal(7522-7643) SV40 ... SV40 poly(A) signal(7522-7643) ...SV40 SV40 poly(A) signal(7522-7643) SV40 ori SV40 ori(7683-7818) SV40 ... SV40 ori(7683-7818) ...SV40 SV40 ori(7683-7818) AmpR promoter AmpR promoter(7818-7889) AmpR ... AmpR promoter(7818-7889) ...AmpR AmpR promoter(7818-7889) AmpR AmpR(7890-8750) AmpR AmpR(7890-8750) ori ori(8921-9509) ori ori(8921-9509) CAP binding site CAP binding site(9797-9818) CAP b... CAP binding site(9797-9818) ...CAP b CAP binding site(9797-9818) lac promoter lac promoter(9833-9863) lac p... lac promoter(9833-9863) ...lac p lac promoter(9833-9863) lac operator lac operator(9871-9887) lac o... lac operator(9871-9887) ...lac o lac operator(9871-9887) M13 rev M13 rev(9895-9911) M13 r... M13 rev(9895-9911) ...M13 r M13 rev(9895-9911) T3 promoter T3 promoter(9932-9950) T3 pr... T3 promoter(9932-9950) ...T3 pr T3 promoter(9932-9950) RSV promoter RSV promoter(9978-10204) RSV p... RSV promoter(9978-10204) ...RSV p RSV promoter(9978-10204) 5' LTR (truncated) 5' LTR (truncated)(10205-10385) 5' LT... 5' LTR (truncated)(10205-10385) ...5' LT 5' LTR (truncated)(10205-10385) HIV-1 Ψ HIV-1 Ψ(10432-10557) HIV-1... HIV-1 Ψ(10432-10557) ...HIV-1 HIV-1 Ψ(10432-10557) RRE RRE(11050-11283) RRE RRE(11050-11283) gp41 peptide gp41 peptide(11468-11512) gp41 ... gp41 peptide(11468-11512) ...gp41 gp41 peptide(11468-11512) Factor Xa site Factor Xa site(7003-7014) Facto... Factor Xa site(7003-7014) ...Facto Factor Xa site(7003-7014) M13 rev M13 rev(9897-9913) M13 r... M13 rev(9897-9913) ...M13 r M13 rev(9897-9913) lac operator lac operator(9873-9889) lac o... lac operator(9873-9889) ...lac o lac operator(9873-9889) tet operator tet operator(222-240) tet o... tet operator(222-240) tet o... tet operator(222-240) tet operator tet operator(258-276) tet o... tet operator(258-276) tet o... tet operator(258-276) tet operator tet operator(293-311) tet o... tet operator(293-311) tet o... tet operator(293-311) tet operator tet operator(329-347) tet o... tet operator(329-347) ...tet o tet operator(329-347) tet operator tet operator(365-383) tet o... tet operator(365-383) ...tet o tet operator(365-383) tet operator tet operator(400-418) tet o... tet operator(400-418) ...tet o tet operator(400-418) tet operator tet operator(436-454) tet o... tet operator(436-454) ...tet o tet operator(436-454) T3 promoter T3 promoter(9934-9952) T3 pr... T3 promoter(9934-9952) ...T3 pr T3 promoter(9934-9952) CAP binding site CAP binding site(9799-9820) CAP b... CAP binding site(9799-9820) ...CAP b CAP binding site(9799-9820) lac promoter lac promoter(9835-9865) lac p... lac promoter(9835-9865) ...lac p lac promoter(9835-9865) nucleoplasmin NLS nucleoplasmin NLS(4772-4819) nucle... nucleoplasmin NLS(4772-4819) nucle... nucleoplasmin NLS(4772-4819) cPPT/CTS cPPT/CTS(43-160) cPPT/... cPPT/CTS(43-160) cPPT/... cPPT/CTS(43-160) HIV-1 Ψ HIV-1 Ψ(10434-10559) HIV-1... HIV-1 Ψ(10434-10559) ...HIV-1 HIV-1 Ψ(10434-10559) SV40 ori SV40 ori(7685-7820) SV40 ... SV40 ori(7685-7820) ...SV40 SV40 ori(7685-7820) RSV promoter RSV promoter(9978-10206) RSV p... RSV promoter(9978-10206) ...RSV p RSV promoter(9978-10206) RRE RRE(11052-11285) RRE RRE(11052-11285) RRE RRE(11052-11285) 3' LTR (ΔU3) 3' LTR (ΔU3)(7219-7452) 3' LT... 3' LTR (ΔU3)(7219-7452) ...3' LT 3' LTR (ΔU3)(7219-7452) tight TRE promoter tight TRE promoter(214-528) tight... tight TRE promoter(214-528) tight... tight TRE promoter(214-528) hPGK promoter hPGK promoter(4829-5339) hPGK ... hPGK promoter(4829-5339) hPGK ... hPGK promoter(4829-5339)