U6 promoter U6 promoter(162-402) U6 pr... U6 promoter(162-402) U6 pr... U6 promoter(162-402) gRNA scaffold gRNA scaffold(931-1006) gRNA ... gRNA scaffold(931-1006) gRNA ... gRNA scaffold(931-1006) cPPT/CTS cPPT/CTS(1062-1179) cPPT/... cPPT/CTS(1062-1179) cPPT/... cPPT/CTS(1062-1179) EF-1α promoter EF-1α promoter(1318-2496) EF-1α... EF-1α promoter(1318-2496) EF-1α intron A EF-1α intron A(1549-2487) EF-1α... EF-1α intron A(1549-2487) PuroR PuroR(2509-3108) PuroR PuroR(2509-3108) WPRE WPRE(3124-3712) WPRE WPRE(3124-3712) Factor Xa site Factor Xa site(3595-3606) Facto... Factor Xa site(3595-3606) Facto... Factor Xa site(3595-3606) 3' LTR (ΔU3) 3' LTR (ΔU3)(3784-4017) 3' LT... 3' LTR (ΔU3)(3784-4017) 3' LT... 3' LTR (ΔU3)(3784-4017) SV40 poly(A) signal SV40 poly(A) signal(4095-4216) SV40 ... SV40 poly(A) signal(4095-4216) SV40 ... SV40 poly(A) signal(4095-4216) SV40 ori SV40 ori(4256-4391) SV40 ... SV40 ori(4256-4391) SV40 ... SV40 ori(4256-4391) T7 promoter T7 promoter(4412-4430) T7 pr... T7 promoter(4412-4430) ...T7 pr T7 promoter(4412-4430) M13 fwd M13 fwd(4440-4456) M13 f... M13 fwd(4440-4456) ...M13 f M13 fwd(4440-4456) f1 ori f1 ori(4598-5053) f1 or... f1 ori(4598-5053) AmpR promoter AmpR promoter(5079-5183) AmpR ... AmpR promoter(5079-5183) ...AmpR AmpR promoter(5079-5183) AmpR AmpR(5184-6044) AmpR AmpR(5184-6044) ori ori(6215-6803) ori ori(6215-6803) CAP binding site CAP binding site(7091-7112) CAP b... CAP binding site(7091-7112) ...CAP b CAP binding site(7091-7112) lac promoter lac promoter(7127-7157) lac p... lac promoter(7127-7157) ...lac p lac promoter(7127-7157) lac operator lac operator(7165-7181) lac o... lac operator(7165-7181) ...lac o lac operator(7165-7181) M13 rev M13 rev(7189-7205) M13 r... M13 rev(7189-7205) ...M13 r M13 rev(7189-7205) T3 promoter T3 promoter(7226-7244) T3 pr... T3 promoter(7226-7244) ...T3 pr T3 promoter(7226-7244) RSV promoter RSV promoter(7272-7498) RSV p... RSV promoter(7272-7498) ...RSV p RSV promoter(7272-7498) 5' LTR (truncated) 5' LTR (truncated)(7499-7679) 5' LT... 5' LTR (truncated)(7499-7679) ...5' LT 5' LTR (truncated)(7499-7679) HIV-1 Ψ HIV-1 Ψ(7726-7851) HIV-1... HIV-1 Ψ(7726-7851) ...HIV-1 HIV-1 Ψ(7726-7851) RRE RRE(8344-8577) RRE RRE(8344-8577) Factor Xa site Factor Xa site(3597-3608) Facto... Factor Xa site(3597-3608) Facto... Factor Xa site(3597-3608) M13 rev M13 rev(7191-7207) M13 r... M13 rev(7191-7207) ...M13 r M13 rev(7191-7207) M13 fwd M13 fwd(4442-4458) M13 f... M13 fwd(4442-4458) ...M13 f M13 fwd(4442-4458) lac operator lac operator(7167-7183) lac o... lac operator(7167-7183) ...lac o lac operator(7167-7183) T7 promoter T7 promoter(4414-4432) T7 pr... T7 promoter(4414-4432) ...T7 pr T7 promoter(4414-4432) T3 promoter T3 promoter(7228-7246) T3 pr... T3 promoter(7228-7246) ...T3 pr T3 promoter(7228-7246) CAP binding site CAP binding site(7093-7114) CAP b... CAP binding site(7093-7114) ...CAP b CAP binding site(7093-7114) lac promoter lac promoter(7129-7159) lac p... lac promoter(7129-7159) ...lac p lac promoter(7129-7159) gRNA scaffold gRNA scaffold(933-1008) gRNA ... gRNA scaffold(933-1008) gRNA ... gRNA scaffold(933-1008) AmpR promoter AmpR promoter(5081-5185) AmpR ... AmpR promoter(5081-5185) ...AmpR AmpR promoter(5081-5185) cPPT/CTS cPPT/CTS(1064-1181) cPPT/... cPPT/CTS(1064-1181) cPPT/... cPPT/CTS(1064-1181) HIV-1 Ψ HIV-1 Ψ(7728-7853) HIV-1... HIV-1 Ψ(7728-7853) ...HIV-1 HIV-1 Ψ(7728-7853) SV40 ori SV40 ori(4258-4393) SV40 ... SV40 ori(4258-4393) SV40 ... SV40 ori(4258-4393) RSV promoter RSV promoter(7272-7500) RSV p... RSV promoter(7272-7500) ...RSV p RSV promoter(7272-7500) RRE RRE(8346-8579) RRE RRE(8346-8579) 3' LTR (ΔU3) 3' LTR (ΔU3)(3786-4019) 3' LT... 3' LTR (ΔU3)(3786-4019) 3' LT... 3' LTR (ΔU3)(3786-4019) U6 promoter U6 promoter(164-404) U6 pr... U6 promoter(164-404) U6 pr... U6 promoter(164-404) WPRE WPRE(3126-3714) WPRE WPRE(3126-3714) EF-1α intron A EF-1α intron A(1551-2489) EF-1α... EF-1α intron A(1551-2489)