U6 promoter U6 promoter(162-402) U6 pr... U6 promoter(162-402) U6 pr... U6 promoter(162-402) gRNA scaffold gRNA scaffold(931-1006) gRNA ... gRNA scaffold(931-1006) gRNA ... gRNA scaffold(931-1006) cPPT/CTS cPPT/CTS(1062-1179) cPPT/... cPPT/CTS(1062-1179) cPPT/... cPPT/CTS(1062-1179) EF-1α promoter EF-1α promoter(1318-2496) EF-1α... EF-1α promoter(1318-2496) EF-1α intron A EF-1α intron A(1549-2487) EF-1α... EF-1α intron A(1549-2487) PuroR PuroR(2509-3105) PuroR PuroR(2509-3105) P2A P2A(3106-3162) P2A P2A(3106-3162) P2A P2A(3106-3162) EGFP EGFP(3163-3879) EGFP EGFP(3163-3879) WPRE WPRE(3897-4485) WPRE WPRE(3897-4485) Factor Xa site Factor Xa site(4368-4379) Facto... Factor Xa site(4368-4379) Facto... Factor Xa site(4368-4379) 3' LTR (ΔU3) 3' LTR (ΔU3)(4557-4790) 3' LT... 3' LTR (ΔU3)(4557-4790) 3' LT... 3' LTR (ΔU3)(4557-4790) SV40 poly(A) signal SV40 poly(A) signal(4868-4989) SV40 ... SV40 poly(A) signal(4868-4989) ...SV40 SV40 poly(A) signal(4868-4989) SV40 ori SV40 ori(5029-5164) SV40 ... SV40 ori(5029-5164) ...SV40 SV40 ori(5029-5164) T7 promoter T7 promoter(5185-5203) T7 pr... T7 promoter(5185-5203) ...T7 pr T7 promoter(5185-5203) M13 fwd M13 fwd(5213-5229) M13 f... M13 fwd(5213-5229) ...M13 f M13 fwd(5213-5229) f1 ori f1 ori(5371-5826) f1 or... f1 ori(5371-5826) ...f1 or f1 ori(5371-5826) AmpR promoter AmpR promoter(5852-5956) AmpR ... AmpR promoter(5852-5956) ...AmpR AmpR promoter(5852-5956) AmpR AmpR(5957-6817) AmpR AmpR(5957-6817) ori ori(6988-7576) ori ori(6988-7576) CAP binding site CAP binding site(7864-7885) CAP b... CAP binding site(7864-7885) ...CAP b CAP binding site(7864-7885) lac promoter lac promoter(7900-7930) lac p... lac promoter(7900-7930) ...lac p lac promoter(7900-7930) lac operator lac operator(7938-7954) lac o... lac operator(7938-7954) ...lac o lac operator(7938-7954) M13 rev M13 rev(7962-7978) M13 r... M13 rev(7962-7978) ...M13 r M13 rev(7962-7978) T3 promoter T3 promoter(7999-8017) T3 pr... T3 promoter(7999-8017) ...T3 pr T3 promoter(7999-8017) RSV promoter RSV promoter(8045-8271) RSV p... RSV promoter(8045-8271) ...RSV p RSV promoter(8045-8271) 5' LTR (truncated) 5' LTR (truncated)(8272-8452) 5' LT... 5' LTR (truncated)(8272-8452) ...5' LT 5' LTR (truncated)(8272-8452) HIV-1 Ψ HIV-1 Ψ(8499-8624) HIV-1... HIV-1 Ψ(8499-8624) ...HIV-1 HIV-1 Ψ(8499-8624) RRE RRE(9117-9350) RRE RRE(9117-9350) gp41 peptide gp41 peptide(9535-9579) gp41 ... gp41 peptide(9535-9579) ...gp41 gp41 peptide(9535-9579) Factor Xa site Factor Xa site(4370-4381) Facto... Factor Xa site(4370-4381) Facto... Factor Xa site(4370-4381) M13 rev M13 rev(7964-7980) M13 r... M13 rev(7964-7980) ...M13 r M13 rev(7964-7980) M13 fwd M13 fwd(5215-5231) M13 f... M13 fwd(5215-5231) ...M13 f M13 fwd(5215-5231) lac operator lac operator(7940-7956) lac o... lac operator(7940-7956) ...lac o lac operator(7940-7956) T7 promoter T7 promoter(5187-5205) T7 pr... T7 promoter(5187-5205) ...T7 pr T7 promoter(5187-5205) T3 promoter T3 promoter(8001-8019) T3 pr... T3 promoter(8001-8019) ...T3 pr T3 promoter(8001-8019) CAP binding site CAP binding site(7866-7887) CAP b... CAP binding site(7866-7887) ...CAP b CAP binding site(7866-7887) lac promoter lac promoter(7902-7932) lac p... lac promoter(7902-7932) ...lac p lac promoter(7902-7932) gRNA scaffold gRNA scaffold(933-1008) gRNA ... gRNA scaffold(933-1008) gRNA ... gRNA scaffold(933-1008) AmpR promoter AmpR promoter(5854-5958) AmpR ... AmpR promoter(5854-5958) ...AmpR AmpR promoter(5854-5958) cPPT/CTS cPPT/CTS(1064-1181) cPPT/... cPPT/CTS(1064-1181) cPPT/... cPPT/CTS(1064-1181) HIV-1 Ψ HIV-1 Ψ(8501-8626) HIV-1... HIV-1 Ψ(8501-8626) ...HIV-1 HIV-1 Ψ(8501-8626) SV40 ori SV40 ori(5031-5166) SV40 ... SV40 ori(5031-5166) ...SV40 SV40 ori(5031-5166) RSV promoter RSV promoter(8045-8273) RSV p... RSV promoter(8045-8273) ...RSV p RSV promoter(8045-8273) RRE RRE(9119-9352) RRE RRE(9119-9352) 3' LTR (ΔU3) 3' LTR (ΔU3)(4559-4792) 3' LT... 3' LTR (ΔU3)(4559-4792) 3' LT... 3' LTR (ΔU3)(4559-4792) U6 promoter U6 promoter(164-404) U6 pr... U6 promoter(164-404) U6 pr... U6 promoter(164-404) WPRE WPRE(3899-4487) WPRE WPRE(3899-4487) EGFP EGFP(3165-3884) EGFP EGFP(3165-3884) EF-1α intron A EF-1α intron A(1551-2489) EF-1α... EF-1α intron A(1551-2489)