CAP binding site CAP binding site(121-142) CAP b... CAP binding site(121-142) CAP b... CAP binding site(121-142) lac promoter lac promoter(157-187) lac p... lac promoter(157-187) lac p... lac promoter(157-187) lac operator lac operator(195-211) lac o... lac operator(195-211) lac o... lac operator(195-211) M13 rev M13 rev(219-235) M13 r... M13 rev(219-235) M13 r... M13 rev(219-235) T3 promoter T3 promoter(256-274) T3 pr... T3 promoter(256-274) T3 pr... T3 promoter(256-274) RSV promoter RSV promoter(302-528) RSV p... RSV promoter(302-528) ...RSV p RSV promoter(302-528) 5' LTR (truncated) 5' LTR (truncated)(529-709) 5' LT... 5' LTR (truncated)(529-709) ...5' LT 5' LTR (truncated)(529-709) HIV-1 Ψ HIV-1 Ψ(756-881) HIV-1... HIV-1 Ψ(756-881) HIV-1... HIV-1 Ψ(756-881) RRE RRE(1374-1607) RRE RRE(1374-1607) gp41 peptide gp41 peptide(1792-1836) gp41 ... gp41 peptide(1792-1836) gp41 ... gp41 peptide(1792-1836) cPPT/CTS cPPT/CTS(2017-2134) cPPT/... cPPT/CTS(2017-2134) cPPT/... cPPT/CTS(2017-2134) EF-1α promoter EF-1α promoter(2273-3451) EF-1α... EF-1α promoter(2273-3451) EF-1α intron A EF-1α intron A(2504-3442) EF-1α... EF-1α intron A(2504-3442) PuroR PuroR(3464-4063) PuroR PuroR(3464-4063) WPRE WPRE(4079-4667) WPRE WPRE(4079-4667) Factor Xa site Factor Xa site(4550-4561) Facto... Factor Xa site(4550-4561) Facto... Factor Xa site(4550-4561) U6 promoter U6 promoter(4823-5063) U6 pr... U6 promoter(4823-5063) U6 pr... U6 promoter(4823-5063) gRNA scaffold gRNA scaffold(6953-7028) gRNA ... gRNA scaffold(6953-7028) ...gRNA gRNA scaffold(6953-7028) 5' LTR (truncated) 5' LTR (truncated)(7081-7261) 5' LT... 5' LTR (truncated)(7081-7261) ...5' LT 5' LTR (truncated)(7081-7261) SV40 poly(A) signal SV40 poly(A) signal(7339-7460) SV40 ... SV40 poly(A) signal(7339-7460) ...SV40 SV40 poly(A) signal(7339-7460) SV40 ori SV40 ori(7500-7635) SV40 ... SV40 ori(7500-7635) ...SV40 SV40 ori(7500-7635) T7 promoter T7 promoter(7656-7674) T7 pr... T7 promoter(7656-7674) ...T7 pr T7 promoter(7656-7674) M13 fwd M13 fwd(7684-7700) M13 f... M13 fwd(7684-7700) ...M13 f M13 fwd(7684-7700) f1 ori f1 ori(7842-8297) f1 or... f1 ori(7842-8297) ...f1 or f1 ori(7842-8297) AmpR promoter AmpR promoter(8323-8427) AmpR ... AmpR promoter(8323-8427) ...AmpR AmpR promoter(8323-8427) AmpR AmpR(8428-9288) AmpR AmpR(8428-9288) ori ori(9459-10047) ori ori(9459-10047) Factor Xa site Factor Xa site(4552-4563) Facto... Factor Xa site(4552-4563) Facto... Factor Xa site(4552-4563) M13 rev M13 rev(221-237) M13 r... M13 rev(221-237) M13 r... M13 rev(221-237) M13 fwd M13 fwd(7686-7702) M13 f... M13 fwd(7686-7702) ...M13 f M13 fwd(7686-7702) lac operator lac operator(197-213) lac o... lac operator(197-213) lac o... lac operator(197-213) T7 promoter T7 promoter(7658-7676) T7 pr... T7 promoter(7658-7676) ...T7 pr T7 promoter(7658-7676) T3 promoter T3 promoter(258-276) T3 pr... T3 promoter(258-276) T3 pr... T3 promoter(258-276) CAP binding site CAP binding site(123-144) CAP b... CAP binding site(123-144) CAP b... CAP binding site(123-144) lac promoter lac promoter(159-189) lac p... lac promoter(159-189) lac p... lac promoter(159-189) gRNA scaffold gRNA scaffold(6955-7030) gRNA ... gRNA scaffold(6955-7030) ...gRNA gRNA scaffold(6955-7030) AmpR promoter AmpR promoter(8325-8429) AmpR ... AmpR promoter(8325-8429) ...AmpR AmpR promoter(8325-8429) cPPT/CTS cPPT/CTS(2019-2136) cPPT/... cPPT/CTS(2019-2136) cPPT/... cPPT/CTS(2019-2136) HIV-1 Ψ HIV-1 Ψ(758-883) HIV-1... HIV-1 Ψ(758-883) HIV-1... HIV-1 Ψ(758-883) SV40 ori SV40 ori(7502-7637) SV40 ... SV40 ori(7502-7637) ...SV40 SV40 ori(7502-7637) RSV promoter RSV promoter(302-530) RSV p... RSV promoter(302-530) ...RSV p RSV promoter(302-530) RRE RRE(1376-1609) RRE RRE(1376-1609) RRE RRE(1376-1609) U6 promoter U6 promoter(4825-5065) U6 pr... U6 promoter(4825-5065) U6 pr... U6 promoter(4825-5065) WPRE WPRE(4081-4669) WPRE WPRE(4081-4669) EF-1α intron A EF-1α intron A(2506-3444) EF-1α... EF-1α intron A(2506-3444)