SV40 NLS SV40 NLS(869-889) SV40 ... SV40 NLS(869-889) SV40 ... SV40 NLS(869-889) Kozak sequence Kozak sequence(892-901) Kozak... Kozak sequence(892-901) Kozak... Kozak sequence(892-901) CMV promoter CMV promoter(920-1123) CMV p... CMV promoter(920-1123) CMV p... CMV promoter(920-1123) CMV enhancer CMV enhancer(1124-1503) CMV e... CMV enhancer(1124-1503) AAV2 ITR (alternate) AAV2 ITR (alternate)(1527-1656) AAV2 ... AAV2 ITR (alternate)(1527-1656) AAV2 ... AAV2 ITR (alternate)(1527-1656) ori ori(1718-2306) ori ori(1718-2306) AmpR AmpR(2477-3337) AmpR AmpR(2477-3337) SaCas9 SaCas9(5135-844) SaCas... SaCas9(5135-844) AmpR promoter AmpR promoter(3338-3442) AmpR ... AmpR promoter(3338-3442) AmpR ... AmpR promoter(3338-3442) f1 ori f1 ori(3724-4179) f1 or... f1 ori(3724-4179) AAV2 ITR AAV2 ITR(4254-4394) AAV2 ... AAV2 ITR(4254-4394) ...AAV2 AAV2 ITR(4254-4394) AAV2 ITR AAV2 ITR(4265-4394) AAV2 ... AAV2 ITR(4265-4394) ...AAV2 AAV2 ITR(4265-4394) Sa gRNA scaffold Sa gRNA scaffold(4408-4483) Sa gR... Sa gRNA scaffold(4408-4483) ...Sa gR Sa gRNA scaffold(4408-4483) U6 promoter U6 promoter(4504-4752) U6 pr... U6 promoter(4504-4752) ...U6 pr U6 promoter(4504-4752) bGH poly(A) signal bGH poly(A) signal(4759-4966) bGH p... bGH poly(A) signal(4759-4966) ...bGH p bGH poly(A) signal(4759-4966) 3xHA 3xHA(5000-5080) 3xHA 3xHA(5000-5080) 3xHA 3xHA(5000-5080) nucleoplasmin NLS nucleoplasmin NLS(5087-5134) nucle... nucleoplasmin NLS(5087-5134) ...nucle nucleoplasmin NLS(5087-5134) nucleoplasmin NLS nucleoplasmin NLS(5089-5136) nucle... nucleoplasmin NLS(5089-5136) ...nucle nucleoplasmin NLS(5089-5136) AmpR promoter AmpR promoter(3340-3444) AmpR ... AmpR promoter(3340-3444) AmpR ... AmpR promoter(3340-3444) AAV2 ITR AAV2 ITR(4256-4396) AAV2 ... AAV2 ITR(4256-4396) ...AAV2 AAV2 ITR(4256-4396) CMV promoter CMV promoter(922-1125) CMV p... CMV promoter(922-1125) CMV p... CMV promoter(922-1125) CMV enhancer CMV enhancer(1126-1505) CMV e... CMV enhancer(1126-1505) CMV e... CMV enhancer(1126-1505) AmpR AmpR(2479-3339) AmpR AmpR(2479-3339)