AAV2 ITR (alternate) AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) CMV enhancer CMV enhancer(154-533) CMV e... CMV enhancer(154-533) CMV e... CMV enhancer(154-533) CMV promoter CMV promoter(534-737) CMV p... CMV promoter(534-737) CMV p... CMV promoter(534-737) Kozak sequence Kozak sequence(756-765) Kozak... Kozak sequence(756-765) Kozak... Kozak sequence(756-765) SV40 NLS SV40 NLS(768-788) SV40 ... SV40 NLS(768-788) SV40 ... SV40 NLS(768-788) SaCas9 SaCas9(813-3968) SaCas... SaCas9(813-3968) nucleoplasmin NLS nucleoplasmin NLS(3969-4016) nucle... nucleoplasmin NLS(3969-4016) nucle... nucleoplasmin NLS(3969-4016) T2A T2A(4017-4070) T2A T2A(4017-4070) T2A T2A(4017-4070) mCherry mCherry(4071-4781) mCher... mCherry(4071-4781) bGH poly(A) signal bGH poly(A) signal(4812-5019) bGH p... bGH poly(A) signal(4812-5019) ...bGH p bGH poly(A) signal(4812-5019) U6 promoter U6 promoter(5027-5267) U6 pr... U6 promoter(5027-5267) ...U6 pr U6 promoter(5027-5267) Sa gRNA scaffold Sa gRNA scaffold(5296-5371) Sa gR... Sa gRNA scaffold(5296-5371) ...Sa gR Sa gRNA scaffold(5296-5371) AAV2 ITR AAV2 ITR(5385-5514) AAV2 ... AAV2 ITR(5385-5514) ...AAV2 AAV2 ITR(5385-5514) AAV2 ITR AAV2 ITR(5385-5525) AAV2 ... AAV2 ITR(5385-5525) ...AAV2 AAV2 ITR(5385-5525) f1 ori f1 ori(5600-6055) f1 or... f1 ori(5600-6055) AmpR promoter AmpR promoter(6337-6441) AmpR ... AmpR promoter(6337-6441) ...AmpR AmpR promoter(6337-6441) AmpR AmpR(6442-7302) AmpR AmpR(6442-7302) ori ori(7473-8061) ori ori(7473-8061) nucleoplasmin NLS nucleoplasmin NLS(3971-4018) nucle... nucleoplasmin NLS(3971-4018) nucle... nucleoplasmin NLS(3971-4018) AmpR promoter AmpR promoter(6339-6443) AmpR ... AmpR promoter(6339-6443) ...AmpR AmpR promoter(6339-6443) AAV2 ITR AAV2 ITR(5387-5527) AAV2 ... AAV2 ITR(5387-5527) ...AAV2 AAV2 ITR(5387-5527) CMV promoter CMV promoter(536-739) CMV p... CMV promoter(536-739) CMV p... CMV promoter(536-739) CMV enhancer CMV enhancer(156-535) CMV e... CMV enhancer(156-535) CMV e... CMV enhancer(156-535) M13 ori M13 ori(5602-5982) M13 o... M13 ori(5602-5982) ...M13 o M13 ori(5602-5982) ori ori(7475-8063) ori ori(7475-8063) AmpR AmpR(6444-7304) AmpR AmpR(6444-7304)