AAV2 ITR (alternate) AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) U6 promoter U6 promoter(145-393) U6 pr... U6 promoter(145-393) U6 pr... U6 promoter(145-393) gRNA scaffold gRNA scaffold(412-487) gRNA ... gRNA scaffold(412-487) gRNA ... gRNA scaffold(412-487) CMV enhancer CMV enhancer(581-884) CMV e... CMV enhancer(581-884) CMV promoter CMV promoter(885-1088) CMV p... CMV promoter(885-1088) CMV p... CMV promoter(885-1088) Kozak sequence Kozak sequence(1127-1136) Kozak... Kozak sequence(1127-1136) Kozak... Kozak sequence(1127-1136) EGFP EGFP(1133-1849) EGFP EGFP(1133-1849) hGH poly(A) signal hGH poly(A) signal(1928-2404) hGH p... hGH poly(A) signal(1928-2404) AAV2 ITR AAV2 ITR(2444-2573) AAV2 ... AAV2 ITR(2444-2573) AAV2 ... AAV2 ITR(2444-2573) AAV2 ITR AAV2 ITR(2444-2584) AAV2 ... AAV2 ITR(2444-2584) AAV2 ... AAV2 ITR(2444-2584) f1 ori f1 ori(2659-3114) f1 or... f1 ori(2659-3114) AmpR promoter AmpR promoter(3396-3500) AmpR ... AmpR promoter(3396-3500) ...AmpR AmpR promoter(3396-3500) AmpR AmpR(3501-4361) AmpR AmpR(3501-4361) ori ori(4532-5120) ori ori(4532-5120) gRNA scaffold gRNA scaffold(414-489) gRNA ... gRNA scaffold(414-489) gRNA ... gRNA scaffold(414-489) AmpR promoter AmpR promoter(3398-3502) AmpR ... AmpR promoter(3398-3502) ...AmpR AmpR promoter(3398-3502) AAV2 ITR AAV2 ITR(2446-2586) AAV2 ... AAV2 ITR(2446-2586) AAV2 ... AAV2 ITR(2446-2586) CMV promoter CMV promoter(887-1090) CMV p... CMV promoter(887-1090) CMV p... CMV promoter(887-1090)