AAV2 ITR (alternate) AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) AAV2 ... AAV2 ITR (alternate)(1-130) U6 promoter U6 promoter(145-393) U6 pr... U6 promoter(145-393) U6 pr... U6 promoter(145-393) gRNA scaffold gRNA scaffold(412-487) gRNA ... gRNA scaffold(412-487) gRNA ... gRNA scaffold(412-487) hSyn promoter hSyn promoter(521-968) hSyn ... hSyn promoter(521-968) Kozak sequence Kozak sequence(1000-1009) Kozak... Kozak sequence(1000-1009) Kozak... Kozak sequence(1000-1009) EGFP EGFP(1006-1722) EGFP EGFP(1006-1722) hGH poly(A) signal hGH poly(A) signal(2039-2515) hGH p... hGH poly(A) signal(2039-2515) AAV2 ITR AAV2 ITR(2555-2684) AAV2 ... AAV2 ITR(2555-2684) AAV2 ... AAV2 ITR(2555-2684) AAV2 ITR AAV2 ITR(2555-2695) AAV2 ... AAV2 ITR(2555-2695) AAV2 ... AAV2 ITR(2555-2695) f1 ori f1 ori(2770-3225) f1 or... f1 ori(2770-3225) AmpR promoter AmpR promoter(3507-3611) AmpR ... AmpR promoter(3507-3611) ...AmpR AmpR promoter(3507-3611) AmpR AmpR(3612-4472) AmpR AmpR(3612-4472) ori ori(4643-5231) ori ori(4643-5231) gRNA scaffold gRNA scaffold(414-489) gRNA ... gRNA scaffold(414-489) gRNA ... gRNA scaffold(414-489) AmpR promoter AmpR promoter(3509-3613) AmpR ... AmpR promoter(3509-3613) ...AmpR AmpR promoter(3509-3613) AAV2 ITR AAV2 ITR(2557-2697) AAV2 ... AAV2 ITR(2557-2697) AAV2 ... AAV2 ITR(2557-2697)