U6 promoter U6 promoter(1-249) U6 pr... U6 promoter(1-249) U6 pr... U6 promoter(1-249) gRNA scaffold gRNA scaffold(268-343) gRNA ... gRNA scaffold(268-343) gRNA ... gRNA scaffold(268-343) U6 promoter U6 promoter(434-682) U6 pr... U6 promoter(434-682) U6 pr... U6 promoter(434-682) gRNA scaffold gRNA scaffold(709-784) gRNA ... gRNA scaffold(709-784) gRNA ... gRNA scaffold(709-784) CMV enhancer CMV enhancer(874-1159) CMV e... CMV enhancer(874-1159) CMV e... CMV enhancer(874-1159) chicken β-actin promoter chicken β-actin promoter(1161-1438) chick... chicken β-actin promoter(1161-1438) chick... chicken β-actin promoter(1161-1438) hybrid intron hybrid intron(1439-1666) hybri... hybrid intron(1439-1666) hybri... hybrid intron(1439-1666) Kozak sequence Kozak sequence(1678-1687) Kozak... Kozak sequence(1678-1687) Kozak... Kozak sequence(1678-1687) 3xFLAG 3xFLAG(1687-1752) 3xFLA... 3xFLAG(1687-1752) 3xFLA... 3xFLAG(1687-1752) SV40 NLS SV40 NLS(1759-1779) SV40 ... SV40 NLS(1759-1779) SV40 ... SV40 NLS(1759-1779) Cas9 Cas9(1804-5904) Cas9 Cas9(1804-5904) nucleoplasmin NLS nucleoplasmin NLS(5905-5952) nucle... nucleoplasmin NLS(5905-5952) ...nucle nucleoplasmin NLS(5905-5952) bGH poly(A) signal bGH poly(A) signal(5986-6193) bGH p... bGH poly(A) signal(5986-6193) ...bGH p bGH poly(A) signal(5986-6193) AAV2 ITR AAV2 ITR(6202-6331) AAV2 ... AAV2 ITR(6202-6331) ...AAV2 AAV2 ITR(6202-6331) AAV2 ITR AAV2 ITR(6202-6342) AAV2 ... AAV2 ITR(6202-6342) ...AAV2 AAV2 ITR(6202-6342) f1 ori f1 ori(6417-6872) f1 or... f1 ori(6417-6872) AmpR promoter AmpR promoter(7154-7258) AmpR ... AmpR promoter(7154-7258) ...AmpR AmpR promoter(7154-7258) AmpR AmpR(7259-8119) AmpR AmpR(7259-8119) ori ori(8290-8878) ori ori(8290-8878) nucleoplasmin NLS nucleoplasmin NLS(5907-5954) nucle... nucleoplasmin NLS(5907-5954) ...nucle nucleoplasmin NLS(5907-5954) gRNA scaffold gRNA scaffold(270-345) gRNA ... gRNA scaffold(270-345) gRNA ... gRNA scaffold(270-345) gRNA scaffold gRNA scaffold(711-786) gRNA ... gRNA scaffold(711-786) gRNA ... gRNA scaffold(711-786) AmpR promoter AmpR promoter(7156-7260) AmpR ... AmpR promoter(7156-7260) ...AmpR AmpR promoter(7156-7260) AAV2 ITR AAV2 ITR(6204-6344) AAV2 ... AAV2 ITR(6204-6344) ...AAV2 AAV2 ITR(6204-6344) chicken β-actin promoter chicken β-actin promoter(1163-1440) chick... chicken β-actin promoter(1163-1440) chick... chicken β-actin promoter(1163-1440) AmpR AmpR(7261-8121) AmpR AmpR(7261-8121)