nucleoplasmin NLS nucleoplasmin NLS(827-874) nucle... nucleoplasmin NLS(827-874) nucle... nucleoplasmin NLS(827-874) T2A T2A(890-943) T2A T2A(890-943) T2A T2A(890-943) EGFP EGFP(944-1657) EGFP EGFP(944-1657) bGH poly(A) signal bGH poly(A) signal(1691-1898) bGH p... bGH poly(A) signal(1691-1898) bGH p... bGH poly(A) signal(1691-1898) AAV2 ITR AAV2 ITR(1907-2036) AAV2 ... AAV2 ITR(1907-2036) AAV2 ... AAV2 ITR(1907-2036) AAV2 ITR AAV2 ITR(1907-2047) AAV2 ... AAV2 ITR(1907-2047) AAV2 ... AAV2 ITR(1907-2047) f1 ori f1 ori(2122-2577) f1 or... f1 ori(2122-2577) f1 or... f1 ori(2122-2577) AmpR promoter AmpR promoter(2859-2963) AmpR ... AmpR promoter(2859-2963) AmpR ... AmpR promoter(2859-2963) AmpR AmpR(2964-3824) AmpR AmpR(2964-3824) Cas9 Cas9(6014-826) Cas9 Cas9(6014-826) ori ori(3995-4583) ori ori(3995-4583) U6 promoter U6 promoter(4645-4893) U6 pr... U6 promoter(4645-4893) ...U6 pr U6 promoter(4645-4893) gRNA scaffold gRNA scaffold(4912-4987) gRNA ... gRNA scaffold(4912-4987) ...gRNA gRNA scaffold(4912-4987) CMV enhancer CMV enhancer(5084-5369) CMV e... CMV enhancer(5084-5369) ...CMV e CMV enhancer(5084-5369) chicken β-actin promoter chicken β-actin promoter(5371-5648) chick... chicken β-actin promoter(5371-5648) ...chick chicken β-actin promoter(5371-5648) hybrid intron hybrid intron(5649-5876) hybri... hybrid intron(5649-5876) ...hybri hybrid intron(5649-5876) Kozak sequence Kozak sequence(5888-5897) Kozak... Kozak sequence(5888-5897) ...Kozak Kozak sequence(5888-5897) 3xFLAG 3xFLAG(5897-5962) 3xFLA... 3xFLAG(5897-5962) ...3xFLA 3xFLAG(5897-5962) SV40 NLS SV40 NLS(5969-5989) SV40 ... SV40 NLS(5969-5989) ...SV40 SV40 NLS(5969-5989) nucleoplasmin NLS nucleoplasmin NLS(829-876) nucle... nucleoplasmin NLS(829-876) nucle... nucleoplasmin NLS(829-876) gRNA scaffold gRNA scaffold(4914-4989) gRNA ... gRNA scaffold(4914-4989) ...gRNA gRNA scaffold(4914-4989) AmpR promoter AmpR promoter(2861-2965) AmpR ... AmpR promoter(2861-2965) AmpR ... AmpR promoter(2861-2965) AAV2 ITR AAV2 ITR(1909-2049) AAV2 ... AAV2 ITR(1909-2049) AAV2 ... AAV2 ITR(1909-2049) chicken β-actin promoter chicken β-actin promoter(5373-5650) chick... chicken β-actin promoter(5373-5650) ...chick chicken β-actin promoter(5373-5650) AmpR AmpR(2966-3826) AmpR AmpR(2966-3826)