5' LTR (truncated) 5' LTR (truncated)(171-351) 5' LT... 5' LTR (truncated)(171-351) 5' LT... 5' LTR (truncated)(171-351) HIV-1 Ψ HIV-1 Ψ(398-523) HIV-1... HIV-1 Ψ(398-523) HIV-1... HIV-1 Ψ(398-523) RRE RRE(1016-1249) RRE RRE(1016-1249) RRE RRE(1016-1249) gp41 peptide gp41 peptide(1434-1478) gp41 ... gp41 peptide(1434-1478) gp41 ... gp41 peptide(1434-1478) cPPT/CTS cPPT/CTS(1776-1893) cPPT/... cPPT/CTS(1776-1893) cPPT/... cPPT/CTS(1776-1893) U6 promoter U6 promoter(1944-2192) U6 pr... U6 promoter(1944-2192) U6 pr... U6 promoter(1944-2192) gRNA scaffold gRNA scaffold(2214-2289) gRNA ... gRNA scaffold(2214-2289) gRNA ... gRNA scaffold(2214-2289) EF-1α core promoter EF-1α core promoter(2373-2584) EF-1α... EF-1α core promoter(2373-2584) EF-1α... EF-1α core promoter(2373-2584) SV40 NLS SV40 NLS(2634-2654) SV40 ... SV40 NLS(2634-2654) SV40 ... SV40 NLS(2634-2654) APOBEC-1 APOBEC-1(2655-3338) APOBE... APOBEC-1(2655-3338) APOBE... APOBEC-1(2655-3338) Cas9(D10A) Cas9(D10A)(3435-7535) Cas9(... Cas9(D10A)(3435-7535) Cas9 Cas9(3435-7535) Cas9 Cas9(3435-7535) CMV promoter CMV promoter(14083-153) CMV p... CMV promoter(14083-153) CMV p... CMV promoter(14083-153) UGI UGI(7566-7814) UGI UGI(7566-7814) UGI UGI(7566-7814) SV40 NLS SV40 NLS(7857-7877) SV40 ... SV40 NLS(7857-7877) ...SV40 SV40 NLS(7857-7877) P2A P2A(7887-7943) P2A P2A(7887-7943) P2A P2A(7887-7943) PuroR PuroR(7944-8540) PuroR PuroR(7944-8540) WPRE WPRE(8556-9144) WPRE WPRE(8556-9144) Factor Xa site Factor Xa site(9027-9038) Facto... Factor Xa site(9027-9038) ...Facto Factor Xa site(9027-9038) 3' LTR (ΔU3) 3' LTR (ΔU3)(9216-9449) 3' LT... 3' LTR (ΔU3)(9216-9449) ...3' LT 3' LTR (ΔU3)(9216-9449) bGH poly(A) signal bGH poly(A) signal(9481-9705) bGH p... bGH poly(A) signal(9481-9705) ...bGH p bGH poly(A) signal(9481-9705) f1 ori f1 ori(9751-10179) f1 or... f1 ori(9751-10179) ...f1 or f1 ori(9751-10179) SV40 promoter SV40 promoter(10193-10522) SV40 ... SV40 promoter(10193-10522) ...SV40 SV40 promoter(10193-10522) SV40 ori SV40 ori(10373-10508) SV40 ... SV40 ori(10373-10508) ...SV40 SV40 ori(10373-10508) EM7 promoter EM7 promoter(10570-10617) EM7 p... EM7 promoter(10570-10617) ...EM7 p EM7 promoter(10570-10617) BleoR BleoR(10636-11010) BleoR BleoR(10636-11010) BleoR BleoR(10636-11010) SV40 poly(A) signal SV40 poly(A) signal(11140-11261) SV40 ... SV40 poly(A) signal(11140-11261) ...SV40 SV40 poly(A) signal(11140-11261) M13 rev M13 rev(11310-11326) M13 r... M13 rev(11310-11326) ...M13 r M13 rev(11310-11326) lac operator lac operator(11334-11350) lac o... lac operator(11334-11350) ...lac o lac operator(11334-11350) lac promoter lac promoter(11358-11388) lac p... lac promoter(11358-11388) ...lac p lac promoter(11358-11388) CAP binding site CAP binding site(11403-11424) CAP b... CAP binding site(11403-11424) ...CAP b CAP binding site(11403-11424) ori ori(11712-12300) ori ori(11712-12300) AmpR AmpR(12471-13331) AmpR AmpR(12471-13331) AmpR promoter AmpR promoter(13332-13436) AmpR ... AmpR promoter(13332-13436) ...AmpR AmpR promoter(13332-13436) CMV enhancer CMV enhancer(13702-14081) CMV e... CMV enhancer(13702-14081) ...CMV e CMV enhancer(13702-14081) Factor Xa site Factor Xa site(9029-9040) Facto... Factor Xa site(9029-9040) ...Facto Factor Xa site(9029-9040) M13 rev M13 rev(11312-11328) M13 r... M13 rev(11312-11328) ...M13 r M13 rev(11312-11328) lac operator lac operator(11336-11352) lac o... lac operator(11336-11352) ...lac o lac operator(11336-11352) CAP binding site CAP binding site(11405-11426) CAP b... CAP binding site(11405-11426) ...CAP b CAP binding site(11405-11426) lac promoter lac promoter(11360-11390) lac p... lac promoter(11360-11390) ...lac p lac promoter(11360-11390) EM7 promoter EM7 promoter(10572-10619) EM7 p... EM7 promoter(10572-10619) ...EM7 p EM7 promoter(10572-10619) AmpR promoter AmpR promoter(13334-13438) AmpR ... AmpR promoter(13334-13438) ...AmpR AmpR promoter(13334-13438) cPPT/CTS cPPT/CTS(1778-1895) cPPT/... cPPT/CTS(1778-1895) cPPT/... cPPT/CTS(1778-1895) HIV-1 Ψ HIV-1 Ψ(400-525) HIV-1... HIV-1 Ψ(400-525) HIV-1... HIV-1 Ψ(400-525) bGH poly(A) signal bGH poly(A) signal(9483-9707) bGH p... bGH poly(A) signal(9483-9707) ...bGH p bGH poly(A) signal(9483-9707) RRE RRE(1018-1251) RRE RRE(1018-1251) RRE RRE(1018-1251) 3' LTR (ΔU3) 3' LTR (ΔU3)(9218-9451) 3' LT... 3' LTR (ΔU3)(9218-9451) ...3' LT 3' LTR (ΔU3)(9218-9451) U6 promoter U6 promoter(1946-2186) U6 pr... U6 promoter(1946-2186) U6 pr... U6 promoter(1946-2186) BleoR BleoR(10638-11012) BleoR BleoR(10638-11012) BleoR BleoR(10638-11012) CMV enhancer CMV enhancer(13704-14083) CMV e... CMV enhancer(13704-14083) ...CMV e CMV enhancer(13704-14083) WPRE WPRE(8558-9146) WPRE WPRE(8558-9146)