CMV enhancer CMV enhancer(238-617) CMV e... CMV enhancer(238-617) CMV e... CMV enhancer(238-617) CMV promoter CMV promoter(619-817) CMV p... CMV promoter(619-817) CMV p... CMV promoter(619-817) 5' LTR (truncated) 5' LTR (truncated)(835-1015) 5' LT... 5' LTR (truncated)(835-1015) 5' LT... 5' LTR (truncated)(835-1015) HIV-1 Ψ HIV-1 Ψ(1062-1187) HIV-1... HIV-1 Ψ(1062-1187) HIV-1... HIV-1 Ψ(1062-1187) RRE RRE(1680-1913) RRE RRE(1680-1913) RRE RRE(1680-1913) gp41 peptide gp41 peptide(2098-2142) gp41 ... gp41 peptide(2098-2142) gp41 ... gp41 peptide(2098-2142) cPPT/CTS cPPT/CTS(2440-2557) cPPT/... cPPT/CTS(2440-2557) cPPT/... cPPT/CTS(2440-2557) U6 promoter U6 promoter(2608-2856) U6 pr... U6 promoter(2608-2856) U6 pr... U6 promoter(2608-2856) gRNA scaffold gRNA scaffold(4738-4813) gRNA ... gRNA scaffold(4738-4813) gRNA ... gRNA scaffold(4738-4813) EF-1α core promoter EF-1α core promoter(4875-5086) EF-1α... EF-1α core promoter(4875-5086) EF-1α... EF-1α core promoter(4875-5086) SV40 NLS SV40 NLS(5136-5156) SV40 ... SV40 NLS(5136-5156) SV40 ... SV40 NLS(5136-5156) APOBEC-1 APOBEC-1(5157-5840) APOBE... APOBEC-1(5157-5840) APOBE... APOBEC-1(5157-5840) Cas9(D10A) Cas9(D10A)(5937-10037) Cas9(... Cas9(D10A)(5937-10037) Cas9 Cas9(5937-10037) Cas9 Cas9(5937-10037) UGI UGI(10068-10316) UGI UGI(10068-10316) UGI UGI(10068-10316) UGI UGI(10347-10595) UGI UGI(10347-10595) UGI UGI(10347-10595) SV40 NLS SV40 NLS(10638-10658) SV40 ... SV40 NLS(10638-10658) ...SV40 SV40 NLS(10638-10658) P2A P2A(10668-10724) P2A P2A(10668-10724) P2A P2A(10668-10724) PuroR PuroR(10725-11321) PuroR PuroR(10725-11321) WPRE WPRE(11337-11925) WPRE WPRE(11337-11925) Factor Xa site Factor Xa site(11808-11819) Facto... Factor Xa site(11808-11819) ...Facto Factor Xa site(11808-11819) 3' LTR (ΔU3) 3' LTR (ΔU3)(11997-12230) 3' LT... 3' LTR (ΔU3)(11997-12230) ...3' LT 3' LTR (ΔU3)(11997-12230) bGH poly(A) signal bGH poly(A) signal(12262-12486) bGH p... bGH poly(A) signal(12262-12486) ...bGH p bGH poly(A) signal(12262-12486) f1 ori f1 ori(12532-12960) f1 or... f1 ori(12532-12960) ...f1 or f1 ori(12532-12960) SV40 promoter SV40 promoter(12974-13303) SV40 ... SV40 promoter(12974-13303) ...SV40 SV40 promoter(12974-13303) SV40 ori SV40 ori(13154-13289) SV40 ... SV40 ori(13154-13289) ...SV40 SV40 ori(13154-13289) EM7 promoter EM7 promoter(13351-13398) EM7 p... EM7 promoter(13351-13398) ...EM7 p EM7 promoter(13351-13398) BleoR BleoR(13417-13791) BleoR BleoR(13417-13791) BleoR BleoR(13417-13791) SV40 poly(A) signal SV40 poly(A) signal(13921-14042) SV40 ... SV40 poly(A) signal(13921-14042) ...SV40 SV40 poly(A) signal(13921-14042) M13 rev M13 rev(14091-14107) M13 r... M13 rev(14091-14107) ...M13 r M13 rev(14091-14107) lac operator lac operator(14115-14131) lac o... lac operator(14115-14131) ...lac o lac operator(14115-14131) lac promoter lac promoter(14139-14169) lac p... lac promoter(14139-14169) ...lac p lac promoter(14139-14169) CAP binding site CAP binding site(14184-14205) CAP b... CAP binding site(14184-14205) ...CAP b CAP binding site(14184-14205) ori ori(14493-15081) ori ori(14493-15081) AmpR AmpR(15252-16112) AmpR AmpR(15252-16112) AmpR promoter AmpR promoter(16113-16217) AmpR ... AmpR promoter(16113-16217) ...AmpR AmpR promoter(16113-16217) Factor Xa site Factor Xa site(11810-11821) Facto... Factor Xa site(11810-11821) ...Facto Factor Xa site(11810-11821) M13 rev M13 rev(14093-14109) M13 r... M13 rev(14093-14109) ...M13 r M13 rev(14093-14109) lac operator lac operator(14117-14133) lac o... lac operator(14117-14133) ...lac o lac operator(14117-14133) CAP binding site CAP binding site(14186-14207) CAP b... CAP binding site(14186-14207) ...CAP b CAP binding site(14186-14207) lac promoter lac promoter(14141-14171) lac p... lac promoter(14141-14171) ...lac p lac promoter(14141-14171) EM7 promoter EM7 promoter(13353-13400) EM7 p... EM7 promoter(13353-13400) ...EM7 p EM7 promoter(13353-13400) gRNA scaffold gRNA scaffold(4740-4815) gRNA ... gRNA scaffold(4740-4815) gRNA ... gRNA scaffold(4740-4815) AmpR promoter AmpR promoter(16115-16219) AmpR ... AmpR promoter(16115-16219) ...AmpR AmpR promoter(16115-16219) cPPT/CTS cPPT/CTS(2442-2559) cPPT/... cPPT/CTS(2442-2559) cPPT/... cPPT/CTS(2442-2559) HIV-1 Ψ HIV-1 Ψ(1064-1189) HIV-1... HIV-1 Ψ(1064-1189) HIV-1... HIV-1 Ψ(1064-1189) bGH poly(A) signal bGH poly(A) signal(12264-12488) bGH p... bGH poly(A) signal(12264-12488) ...bGH p bGH poly(A) signal(12264-12488) RRE RRE(1682-1915) RRE RRE(1682-1915) RRE RRE(1682-1915) 3' LTR (ΔU3) 3' LTR (ΔU3)(11999-12232) 3' LT... 3' LTR (ΔU3)(11999-12232) ...3' LT 3' LTR (ΔU3)(11999-12232) U6 promoter U6 promoter(2610-2850) U6 pr... U6 promoter(2610-2850) U6 pr... U6 promoter(2610-2850) BleoR BleoR(13419-13793) BleoR BleoR(13419-13793) BleoR BleoR(13419-13793) CMV enhancer CMV enhancer(240-619) CMV e... CMV enhancer(240-619) CMV e... CMV enhancer(240-619) WPRE WPRE(11339-11927) WPRE WPRE(11339-11927)