U6 promoter U6 promoter(94-342) U6 pr... U6 promoter(94-342) U6 pr... U6 promoter(94-342) CMV enhancer CMV enhancer(514-817) CMV e... CMV enhancer(514-817) CMV e... CMV enhancer(514-817) chimeric intron chimeric intron(1095-2111) chime... chimeric intron(1095-2111) SV40 NLS SV40 NLS(2215-2235) SV40 ... SV40 NLS(2215-2235) SV40 ... SV40 NLS(2215-2235) St1Cas9 St1Cas9(3436-6795) St1Ca... St1Cas9(3436-6795) SV40 NLS SV40 NLS(6808-6828) SV40 ... SV40 NLS(6808-6828) ...SV40 SV40 NLS(6808-6828) β-globin poly(A) signal β-globin poly(A) signal(6982-7037) β-glo... β-globin poly(A) signal(6982-7037) ...β-glo β-globin poly(A) signal(6982-7037) M13 rev M13 rev(7398-7414) M13 r... M13 rev(7398-7414) ...M13 r M13 rev(7398-7414) lac operator lac operator(7422-7438) lac o... lac operator(7422-7438) ...lac o lac operator(7422-7438) lac promoter lac promoter(7446-7476) lac p... lac promoter(7446-7476) ...lac p lac promoter(7446-7476) CAP binding site CAP binding site(7491-7512) CAP b... CAP binding site(7491-7512) ...CAP b CAP binding site(7491-7512) SV40 promoter SV40 promoter(7571-7766) SV40 ... SV40 promoter(7571-7766) ...SV40 SV40 promoter(7571-7766) SV40 ori SV40 ori(7617-7752) SV40 ... SV40 ori(7617-7752) ...SV40 SV40 ori(7617-7752) SV40 poly(A) signal SV40 poly(A) signal(7772-7906) SV40 ... SV40 poly(A) signal(7772-7906) ...SV40 SV40 poly(A) signal(7772-7906) ori ori(8145-8733) ori ori(8145-8733) AmpR AmpR(8904-9764) AmpR AmpR(8904-9764) AmpR promoter AmpR promoter(9765-9869) AmpR ... AmpR promoter(9765-9869) ...AmpR AmpR promoter(9765-9869) M13 rev M13 rev(7400-7416) M13 r... M13 rev(7400-7416) ...M13 r M13 rev(7400-7416) lac operator lac operator(7424-7440) lac o... lac operator(7424-7440) ...lac o lac operator(7424-7440) CAP binding site CAP binding site(7493-7514) CAP b... CAP binding site(7493-7514) ...CAP b CAP binding site(7493-7514) lac promoter lac promoter(7448-7478) lac p... lac promoter(7448-7478) ...lac p lac promoter(7448-7478) β-globin poly(A) signal β-globin poly(A) signal(6984-7039) β-glo... β-globin poly(A) signal(6984-7039) ...β-glo β-globin poly(A) signal(6984-7039) AmpR promoter AmpR promoter(9767-9871) AmpR ... AmpR promoter(9767-9871) ...AmpR AmpR promoter(9767-9871) SV40 poly(A) signal SV40 poly(A) signal(7774-7908) SV40 ... SV40 poly(A) signal(7774-7908) ...SV40 SV40 poly(A) signal(7774-7908) SV40 ori SV40 ori(7619-7754) SV40 ... SV40 ori(7619-7754) ...SV40 SV40 ori(7619-7754) U6 promoter U6 promoter(96-336) U6 pr... U6 promoter(96-336) U6 pr... U6 promoter(96-336)