SK primer SK primer(3056-3072) SK pr... SK primer(3056-3072) SK pr... SK primer(3056-3072) f1 ori f1 ori(3157-3612) f1 or... f1 ori(3157-3612) AmpR promoter AmpR promoter(3894-3998) AmpR ... AmpR promoter(3894-3998) ...AmpR AmpR promoter(3894-3998) AmpR AmpR(3999-4859) AmpR AmpR(3999-4859) ori ori(5030-5618) ori ori(5030-5618) gRNA scaffold gRNA scaffold(6192-6267) gRNA ... gRNA scaffold(6192-6267) ...gRNA gRNA scaffold(6192-6267) SK primer SK primer(3058-3074) SK pr... SK primer(3058-3074) SK pr... SK primer(3058-3074) gRNA scaffold gRNA scaffold(6194-6269) gRNA ... gRNA scaffold(6194-6269) ...gRNA gRNA scaffold(6194-6269) AmpR promoter AmpR promoter(3896-4000) AmpR ... AmpR promoter(3896-4000) ...AmpR AmpR promoter(3896-4000) M13 ori M13 ori(3159-3539) M13 o... M13 ori(3159-3539) ...M13 o M13 ori(3159-3539) ori ori(5032-5620) ori ori(5032-5620) AmpR AmpR(4001-4861) AmpR AmpR(4001-4861)