M13 fwd M13 fwd(3-19) M13 f... M13 fwd(3-19) M13 f... M13 fwd(3-19) gRNA scaffold gRNA scaffold(64-139) gRNA ... gRNA scaffold(64-139) gRNA ... gRNA scaffold(64-139) T3 promoter T3 promoter(157-175) T3 pr... T3 promoter(157-175) T3 pr... T3 promoter(157-175) M13 rev M13 rev(196-212) M13 r... M13 rev(196-212) M13 r... M13 rev(196-212) ori ori(379-967) ori ori(379-967) NeoR/KanR NeoR/KanR(1141-1935) NeoR/... NeoR/KanR(1141-1935) AmpR promoter AmpR promoter(1936-2040) AmpR ... AmpR promoter(1936-2040) ...AmpR AmpR promoter(1936-2040) M13 rev M13 rev(198-214) M13 r... M13 rev(198-214) M13 r... M13 rev(198-214) M13 fwd M13 fwd(5-21) M13 f... M13 fwd(5-21) M13 f... M13 fwd(5-21) T3 promoter T3 promoter(159-177) T3 pr... T3 promoter(159-177) T3 pr... T3 promoter(159-177) gRNA scaffold gRNA scaffold(66-141) gRNA ... gRNA scaffold(66-141) gRNA ... gRNA scaffold(66-141) AmpR promoter AmpR promoter(1938-2042) AmpR ... AmpR promoter(1938-2042) ...AmpR AmpR promoter(1938-2042)