3xFLAG 3xFLAG(514-579) 3xFLA... 3xFLAG(514-579) 3xFLA... 3xFLAG(514-579) SV40 NLS SV40 NLS(586-606) SV40 ... SV40 NLS(586-606) SV40 ... SV40 NLS(586-606) Cas9 Cas9(631-4731) Cas9 Cas9(631-4731) nucleoplasmin NLS nucleoplasmin NLS(4732-4779) nucle... nucleoplasmin NLS(4732-4779) ...nucle nucleoplasmin NLS(4732-4779) bGH poly(A) signal bGH poly(A) signal(4813-5020) bGH p... bGH poly(A) signal(4813-5020) ...bGH p bGH poly(A) signal(4813-5020) AAV2 ITR AAV2 ITR(5029-5158) AAV2 ... AAV2 ITR(5029-5158) ...AAV2 AAV2 ITR(5029-5158) AAV2 ITR AAV2 ITR(5029-5169) AAV2 ... AAV2 ITR(5029-5169) ...AAV2 AAV2 ITR(5029-5169) f1 ori f1 ori(5244-5699) f1 or... f1 ori(5244-5699) AmpR promoter AmpR promoter(5981-6085) AmpR ... AmpR promoter(5981-6085) ...AmpR AmpR promoter(5981-6085) AmpR AmpR(6086-6946) AmpR AmpR(6086-6946) ori ori(7117-7705) ori ori(7117-7705) gRNA scaffold gRNA scaffold(8279-8354) gRNA ... gRNA scaffold(8279-8354) ...gRNA gRNA scaffold(8279-8354) nucleoplasmin NLS nucleoplasmin NLS(4734-4781) nucle... nucleoplasmin NLS(4734-4781) ...nucle nucleoplasmin NLS(4734-4781) gRNA scaffold gRNA scaffold(8281-8356) gRNA ... gRNA scaffold(8281-8356) ...gRNA gRNA scaffold(8281-8356) AmpR promoter AmpR promoter(5983-6087) AmpR ... AmpR promoter(5983-6087) ...AmpR AmpR promoter(5983-6087) AAV2 ITR AAV2 ITR(5031-5171) AAV2 ... AAV2 ITR(5031-5171) ...AAV2 AAV2 ITR(5031-5171) M13 ori M13 ori(5246-5626) M13 o... M13 ori(5246-5626) ...M13 o M13 ori(5246-5626) ori ori(7119-7707) ori ori(7119-7707) AmpR AmpR(6088-6948) AmpR AmpR(6088-6948)