RB T-DNA repeat RB T-DNA repeat(21-45) RB T-... RB T-DNA repeat(21-45) RB T-... RB T-DNA repeat(21-45) M13 fwd M13 fwd(248-264) M13 f... M13 fwd(248-264) M13 f... M13 fwd(248-264) SmR SmR(977-1768) SmR SmR(977-1768) gRNA scaffold gRNA scaffold(1920-1995) gRNA ... gRNA scaffold(1920-1995) gRNA ... gRNA scaffold(1920-1995) CaMV 35S promoter CaMV 35S promoter(2609-2953) CaMV ... CaMV 35S promoter(2609-2953) CaMV ... CaMV 35S promoter(2609-2953) 3xFLAG 3xFLAG(3106-3171) 3xFLA... 3xFLAG(3106-3171) 3xFLA... 3xFLAG(3106-3171) SV40 NLS SV40 NLS(3178-3198) SV40 ... SV40 NLS(3178-3198) SV40 ... SV40 NLS(3178-3198) Cas9 Cas9(3223-7323) Cas9 Cas9(3223-7323) Shine-Dalgarno sequence Shine-Dalgarno sequence(7236-7244) Shine... Shine-Dalgarno sequence(7236-7244) Shine... Shine-Dalgarno sequence(7236-7244) nucleoplasmin NLS nucleoplasmin NLS(7324-7371) nucle... nucleoplasmin NLS(7324-7371) nucle... nucleoplasmin NLS(7324-7371) M13 rev M13 rev(8028-8044) M13 r... M13 rev(8028-8044) M13 r... M13 rev(8028-8044) lac operator lac operator(8052-8068) lac o... lac operator(8052-8068) lac o... lac operator(8052-8068) lac promoter lac promoter(8076-8106) lac p... lac promoter(8076-8106) lac p... lac promoter(8076-8106) CAP binding site CAP binding site(8121-8142) CAP b... CAP binding site(8121-8142) CAP b... CAP binding site(8121-8142) CaMV 35S promoter (enhanced) CaMV 35S promoter (enhanced)(8333-9009) CaMV ... CaMV 35S promoter (enhanced)(8333-9009) ...CaMV CaMV 35S promoter (enhanced)(8333-9009) HygR HygR(9076-10101) HygR HygR(9076-10101) CaMV poly(A) signal CaMV poly(A) signal(10142-10316) CaMV ... CaMV poly(A) signal(10142-10316) ...CaMV CaMV poly(A) signal(10142-10316) LB T-DNA repeat LB T-DNA repeat(10394-10418) LB T-... LB T-DNA repeat(10394-10418) ...LB T- LB T-DNA repeat(10394-10418) KanR KanR(10843-11637) KanR KanR(10843-11637) ori ori(11724-12312) ori ori(11724-12312) bom bom(12498-12638) bom bom(12498-12638) bom bom(12498-12638) pVS1 oriV pVS1 oriV(12982-13176) pVS1 ... pVS1 oriV(12982-13176) ...pVS1 pVS1 oriV(12982-13176) pVS1 RepA pVS1 RepA(13242-14315) pVS1 ... pVS1 RepA(13242-14315) pVS1 StaA pVS1 StaA(14744-15373) pVS1 ... pVS1 StaA(14744-15373) ...pVS1 pVS1 StaA(14744-15373) M13 rev M13 rev(8030-8046) M13 r... M13 rev(8030-8046) M13 r... M13 rev(8030-8046) M13 fwd M13 fwd(250-266) M13 f... M13 fwd(250-266) M13 f... M13 fwd(250-266) lac operator lac operator(8054-8070) lac o... lac operator(8054-8070) lac o... lac operator(8054-8070) CAP binding site CAP binding site(8123-8144) CAP b... CAP binding site(8123-8144) CAP b... CAP binding site(8123-8144) LB T-DNA repeat LB T-DNA repeat(10398-10420) LB T-... LB T-DNA repeat(10398-10420) ...LB T- LB T-DNA repeat(10398-10420) RB T-DNA repeat RB T-DNA repeat(23-47) RB T-... RB T-DNA repeat(23-47) RB T-... RB T-DNA repeat(23-47) LB T-DNA repeat LB T-DNA repeat(10396-10420) LB T-... LB T-DNA repeat(10396-10420) ...LB T- LB T-DNA repeat(10396-10420) lac promoter lac promoter(8078-8108) lac p... lac promoter(8078-8108) lac p... lac promoter(8078-8108) gRNA scaffold gRNA scaffold(1922-1997) gRNA ... gRNA scaffold(1922-1997) gRNA ... gRNA scaffold(1922-1997) bom bom(12500-12640) bom bom(12500-12640) bom bom(12500-12640) CaMV poly(A) signal CaMV poly(A) signal(10144-10318) CaMV ... CaMV poly(A) signal(10144-10318) ...CaMV CaMV poly(A) signal(10144-10318) ori ori(11726-12314) ori ori(11726-12314) pVS1 StaA pVS1 StaA(14746-15375) pVS1 ... pVS1 StaA(14746-15375) ...pVS1 pVS1 StaA(14746-15375) KanR KanR(10845-11639) KanR KanR(10845-11639) pVS1 RepA pVS1 RepA(13244-14317) pVS1 ... pVS1 RepA(13244-14317)