nucleoplasmin NLS nucleoplasmin NLS(28-75) nucle... nucleoplasmin NLS(28-75) nucle... nucleoplasmin NLS(28-75) attB2 attB2(108-132) attB2 attB2(108-132) attB2 attB2(108-132) FLAG FLAG(143-166) FLAG FLAG(143-166) FLAG FLAG(143-166) NOS terminator NOS terminator(188-440) NOS t... NOS terminator(188-440) NOS t... NOS terminator(188-440) M13 fwd M13 fwd(449-465) M13 f... M13 fwd(449-465) M13 f... M13 fwd(449-465) RB T-DNA repeat RB T-DNA repeat(668-692) RB T-... RB T-DNA repeat(668-692) ...RB T- RB T-DNA repeat(668-692) pVS1 StaA pVS1 StaA(1993-2622) pVS1 ... pVS1 StaA(1993-2622) pVS1 ... pVS1 StaA(1993-2622) pVS1 RepA pVS1 RepA(3051-4124) pVS1 ... pVS1 RepA(3051-4124) pVS1 oriV pVS1 oriV(4190-4384) pVS1 ... pVS1 oriV(4190-4384) pVS1 ... pVS1 oriV(4190-4384) bom bom(4728-4868) bom bom(4728-4868) bom bom(4728-4868) ori ori(5054-5642) ori ori(5054-5642) Cas9 Cas9(11815-27) Cas9 Cas9(11815-27) KanR KanR(5729-6523) KanR KanR(5729-6523) LB T-DNA repeat LB T-DNA repeat(6948-6972) LB T-... LB T-DNA repeat(6948-6972) LB T-... LB T-DNA repeat(6948-6972) CaMV poly(A) signal CaMV poly(A) signal(7050-7224) CaMV ... CaMV poly(A) signal(7050-7224) CaMV ... CaMV poly(A) signal(7050-7224) HygR HygR(7265-8290) HygR HygR(7265-8290) CaMV 35S promoter (enhanced) CaMV 35S promoter (enhanced)(8357-9033) CaMV ... CaMV 35S promoter (enhanced)(8357-9033) ...CaMV CaMV 35S promoter (enhanced)(8357-9033) CAP binding site CAP binding site(9224-9245) CAP b... CAP binding site(9224-9245) ...CAP b CAP binding site(9224-9245) lac promoter lac promoter(9260-9290) lac p... lac promoter(9260-9290) ...lac p lac promoter(9260-9290) lac operator lac operator(9298-9314) lac o... lac operator(9298-9314) ...lac o lac operator(9298-9314) M13 rev M13 rev(9322-9338) M13 r... M13 rev(9322-9338) ...M13 r M13 rev(9322-9338) OsU3 promoter OsU3 promoter(9356-9736) OsU3 ... OsU3 promoter(9356-9736) ...OsU3 OsU3 promoter(9356-9736) gRNA scaffold gRNA scaffold(9753-9828) gRNA ... gRNA scaffold(9753-9828) ...gRNA gRNA scaffold(9753-9828) Ubi promoter Ubi promoter(9909-11618) Ubi p... Ubi promoter(9909-11618) intron intron(10657-11618) intro... intron(10657-11618) ...intro intron(10657-11618) attB1 attB1(11629-11653) attB1 attB1(11629-11653) attB1 attB1(11629-11653) 3xFLAG 3xFLAG(11698-11763) 3xFLA... 3xFLAG(11698-11763) ...3xFLA 3xFLAG(11698-11763) SV40 NLS SV40 NLS(11770-11790) SV40 ... SV40 NLS(11770-11790) ...SV40 SV40 NLS(11770-11790) enterokinase site enterokinase site(154-168) enter... enterokinase site(154-168) enter... enterokinase site(154-168) M13 rev M13 rev(9324-9340) M13 r... M13 rev(9324-9340) ...M13 r M13 rev(9324-9340) M13 fwd M13 fwd(451-467) M13 f... M13 fwd(451-467) M13 f... M13 fwd(451-467) lac operator lac operator(9300-9316) lac o... lac operator(9300-9316) ...lac o lac operator(9300-9316) attB2 attB2(113-133) attB2 attB2(113-133) attB2 attB2(113-133) attB1 attB1(11632-11652) attB1 attB1(11632-11652) attB1 attB1(11632-11652) CAP binding site CAP binding site(9226-9247) CAP b... CAP binding site(9226-9247) ...CAP b CAP binding site(9226-9247) LB T-DNA repeat LB T-DNA repeat(6950-6972) LB T-... LB T-DNA repeat(6950-6972) LB T-... LB T-DNA repeat(6950-6972) RB T-DNA repeat RB T-DNA repeat(670-694) RB T-... RB T-DNA repeat(670-694) ...RB T- RB T-DNA repeat(670-694) LB T-DNA repeat LB T-DNA repeat(6950-6974) LB T-... LB T-DNA repeat(6950-6974) LB T-... LB T-DNA repeat(6950-6974) lac promoter lac promoter(9262-9292) lac p... lac promoter(9262-9292) ...lac p lac promoter(9262-9292) nucleoplasmin NLS nucleoplasmin NLS(30-77) nucle... nucleoplasmin NLS(30-77) nucle... nucleoplasmin NLS(30-77) gRNA scaffold gRNA scaffold(9755-9830) gRNA ... gRNA scaffold(9755-9830) ...gRNA gRNA scaffold(9755-9830) bom bom(4730-4870) bom bom(4730-4870) bom bom(4730-4870) CaMV poly(A) signal CaMV poly(A) signal(7052-7226) CaMV ... CaMV poly(A) signal(7052-7226) CaMV ... CaMV poly(A) signal(7052-7226) NOS terminator NOS terminator(190-442) NOS t... NOS terminator(190-442) NOS t... NOS terminator(190-442) ori ori(5056-5644) ori ori(5056-5644) pVS1 StaA pVS1 StaA(1995-2624) pVS1 ... pVS1 StaA(1995-2624) pVS1 ... pVS1 StaA(1995-2624) KanR KanR(5731-6525) KanR KanR(5731-6525) pVS1 RepA pVS1 RepA(3053-4126) pVS1 ... pVS1 RepA(3053-4126)