FRT FRT(580-627) FRT FRT(580-627) FRT FRT(580-627) 2μ ori 2μ ori(248-1590) 2μ or... 2μ ori(248-1590) AmpR promoter AmpR promoter(1617-1721) AmpR ... AmpR promoter(1617-1721) AmpR ... AmpR promoter(1617-1721) AmpR AmpR(1722-2582) AmpR AmpR(1722-2582) ori ori(2753-3341) ori ori(2753-3341) SNR52 promoter SNR52 promoter(3378-3646) SNR52... SNR52 promoter(3378-3646) SNR52... SNR52 promoter(3378-3646) gRNA scaffold gRNA scaffold(3670-3744) gRNA ... gRNA scaffold(3670-3744) gRNA ... gRNA scaffold(3670-3744) SUP4 terminator SUP4 terminator(3749-3768) SUP4 ... SUP4 terminator(3749-3768) SUP4 ... SUP4 terminator(3749-3768) CYC1 terminator CYC1 terminator(3789-4036) CYC1 ... CYC1 terminator(3789-4036) CYC1 ... CYC1 terminator(3789-4036) SV40 NLS SV40 NLS(4052-4072) SV40 ... SV40 NLS(4052-4072) SV40 ... SV40 NLS(4052-4072) Cas9 Cas9(4085-8188) Cas9 Cas9(4085-8188) TEF terminator TEF terminator(8877-9074) TEF t... TEF terminator(8877-9074) ...TEF t TEF terminator(8877-9074) KanR KanR(9080-9889) KanR KanR(9080-9889) kanMX kanMX(8877-10233) kanMX kanMX(8877-10233) TEF promoter TEF promoter(9890-10233) TEF p... TEF promoter(9890-10233) ...TEF p TEF promoter(9890-10233) FRT (minimal) FRT (minimal)(596-629) FRT (... FRT (minimal)(596-629) FRT (... FRT (minimal)(596-629) FRT FRT(582-629) FRT FRT(582-629) FRT FRT(582-629) AmpR promoter AmpR promoter(1619-1723) AmpR ... AmpR promoter(1619-1723) AmpR ... AmpR promoter(1619-1723) TEF terminator TEF terminator(8879-9076) TEF t... TEF terminator(8879-9076) ...TEF t TEF terminator(8879-9076) CYC1 terminator CYC1 terminator(3791-4038) CYC1 ... CYC1 terminator(3791-4038) CYC1 ... CYC1 terminator(3791-4038) TEF promoter TEF promoter(9892-10235) TEF p... TEF promoter(9892-10235) ...TEF p TEF promoter(9892-10235) ori ori(2755-3343) ori ori(2755-3343) KanR KanR(9082-9891) KanR KanR(9082-9891) 2μ ori 2μ ori(250-1592) 2μ or... 2μ ori(250-1592) kanMX kanMX(8879-10235) kanMX kanMX(8879-10235)