CMV promoter CMV promoter(132-335) CMV p... CMV promoter(132-335) CMV p... CMV promoter(132-335) T7 promoter T7 promoter(377-395) T7 pr... T7 promoter(377-395) T7 pr... T7 promoter(377-395) Kozak sequence Kozak sequence(403-412) Kozak... Kozak sequence(403-412) Kozak... Kozak sequence(403-412) Cas9 Cas9(409-4512) Cas9 Cas9(409-4512) CMV enhancer CMV enhancer(7366-131) CMV e... CMV enhancer(7366-131) SV40 NLS SV40 NLS(4519-4539) SV40 ... SV40 NLS(4519-4539) ...SV40 SV40 NLS(4519-4539) 3xFLAG 3xFLAG(4546-4611) 3xFLA... 3xFLAG(4546-4611) ...3xFLA 3xFLAG(4546-4611) 6xHis 6xHis(4629-4646) 6xHis 6xHis(4629-4646) 6xHis 6xHis(4629-4646) bGH poly(A) signal bGH poly(A) signal(4675-4899) bGH p... bGH poly(A) signal(4675-4899) ...bGH p bGH poly(A) signal(4675-4899) M13 rev M13 rev(4970-4986) M13 r... M13 rev(4970-4986) ...M13 r M13 rev(4970-4986) lac operator lac operator(4994-5010) lac o... lac operator(4994-5010) ...lac o lac operator(4994-5010) lac promoter lac promoter(5018-5048) lac p... lac promoter(5018-5048) ...lac p lac promoter(5018-5048) CAP binding site CAP binding site(5063-5084) CAP b... CAP binding site(5063-5084) ...CAP b CAP binding site(5063-5084) ori ori(5372-5960) ori ori(5372-5960) AmpR AmpR(6131-6991) AmpR AmpR(6131-6991) AmpR promoter AmpR promoter(6992-7096) AmpR ... AmpR promoter(6992-7096) ...AmpR AmpR promoter(6992-7096) enterokinase site enterokinase site(4599-4613) enter... enterokinase site(4599-4613) ...enter enterokinase site(4599-4613) M13 rev M13 rev(4972-4988) M13 r... M13 rev(4972-4988) ...M13 r M13 rev(4972-4988) lac operator lac operator(4996-5012) lac o... lac operator(4996-5012) ...lac o lac operator(4996-5012) T7 promoter T7 promoter(379-397) T7 pr... T7 promoter(379-397) T7 pr... T7 promoter(379-397) CAP binding site CAP binding site(5065-5086) CAP b... CAP binding site(5065-5086) ...CAP b CAP binding site(5065-5086) lac promoter lac promoter(5020-5050) lac p... lac promoter(5020-5050) ...lac p lac promoter(5020-5050) AmpR promoter AmpR promoter(6994-7098) AmpR ... AmpR promoter(6994-7098) ...AmpR AmpR promoter(6994-7098) CMV promoter CMV promoter(134-337) CMV p... CMV promoter(134-337) CMV p... CMV promoter(134-337) bGH poly(A) signal bGH poly(A) signal(4677-4901) bGH p... bGH poly(A) signal(4677-4901) ...bGH p bGH poly(A) signal(4677-4901)