M13 fwd M13 fwd(10-26) M13 f... M13 fwd(10-26) M13 f... M13 fwd(10-26) gRNA scaffold gRNA scaffold(208-283) gRNA ... gRNA scaffold(208-283) gRNA ... gRNA scaffold(208-283) T7 promoter T7 promoter(305-323) T7 pr... T7 promoter(305-323) T7 pr... T7 promoter(305-323) M13 rev M13 rev(452-468) M13 r... M13 rev(452-468) M13 r... M13 rev(452-468) tonB terminator tonB terminator(516-547) tonB ... tonB terminator(516-547) tonB ... tonB terminator(516-547) cat promoter cat promoter(548-650) cat p... cat promoter(548-650) cat p... cat promoter(548-650) KanR KanR(651-1466) KanR KanR(651-1466) T7Te terminator T7Te terminator(1490-1517) T7Te ... T7Te terminator(1490-1517) ...T7Te T7Te terminator(1490-1517) ori ori(1529-2116) ori ori(1529-2116) M13 rev M13 rev(454-470) M13 r... M13 rev(454-470) M13 r... M13 rev(454-470) M13 fwd M13 fwd(12-28) M13 f... M13 fwd(12-28) M13 f... M13 fwd(12-28) T7 promoter T7 promoter(307-325) T7 pr... T7 promoter(307-325) T7 pr... T7 promoter(307-325) T7Te terminator T7Te terminator(1492-1519) T7Te ... T7Te terminator(1492-1519) ...T7Te T7Te terminator(1492-1519) tonB terminator tonB terminator(518-549) tonB ... tonB terminator(518-549) tonB ... tonB terminator(518-549) gRNA scaffold gRNA scaffold(210-285) gRNA ... gRNA scaffold(210-285) gRNA ... gRNA scaffold(210-285) cat promoter cat promoter(550-652) cat p... cat promoter(550-652) cat p... cat promoter(550-652)