AmpR promoter AmpR promoter(417-521) AmpR ... AmpR promoter(417-521) AmpR ... AmpR promoter(417-521) f1 ori f1 ori(599-1054) f1 or... f1 ori(599-1054) f1 or... f1 ori(599-1054) M13 fwd M13 fwd(1195-1211) M13 f... M13 fwd(1195-1211) M13 f... M13 fwd(1195-1211) T7 promoter T7 promoter(1218-1236) T7 pr... T7 promoter(1218-1236) T7 pr... T7 promoter(1218-1236) 5X UAS 5X UAS(1895-1989) 5X UA... 5X UAS(1895-1989) 5X UA... 5X UAS(1895-1989) hsp70 promoter hsp70 promoter(2008-2246) hsp70... hsp70 promoter(2008-2246) hsp70... hsp70 promoter(2008-2246) Kozak sequence Kozak sequence(2261-2270) Kozak... Kozak sequence(2261-2270) Kozak... Kozak sequence(2261-2270) SV40 NLS SV40 NLS(2279-2299) SV40 ... SV40 NLS(2279-2299) SV40 ... SV40 NLS(2279-2299) Cas9 Cas9(2306-6406) Cas9 Cas9(2306-6406) AmpR AmpR(9539-416) AmpR AmpR(9539-416) Shine-Dalgarno sequence Shine-Dalgarno sequence(6319-6327) Shine... Shine-Dalgarno sequence(6319-6327) ...Shine Shine-Dalgarno sequence(6319-6327) SV40 NLS SV40 NLS(6422-6442) SV40 ... SV40 NLS(6422-6442) ...SV40 SV40 NLS(6422-6442) T2A T2A(6458-6511) T2A T2A(6458-6511) T2A T2A(6458-6511) Cre Cre(6512-7543) Cre Cre(6512-7543) SP6 promoter SP6 promoter(8342-8360) SP6 p... SP6 promoter(8342-8360) ...SP6 p SP6 promoter(8342-8360) M13 rev M13 rev(8378-8394) M13 r... M13 rev(8378-8394) ...M13 r M13 rev(8378-8394) lac operator lac operator(8402-8418) lac o... lac operator(8402-8418) ...lac o lac operator(8402-8418) lac promoter lac promoter(8426-8456) lac p... lac promoter(8426-8456) ...lac p lac promoter(8426-8456) CAP binding site CAP binding site(8471-8492) CAP b... CAP binding site(8471-8492) ...CAP b CAP binding site(8471-8492) ori ori(8780-9368) ori ori(8780-9368) M13 rev M13 rev(8380-8396) M13 r... M13 rev(8380-8396) ...M13 r M13 rev(8380-8396) M13 fwd M13 fwd(1197-1213) M13 f... M13 fwd(1197-1213) M13 f... M13 fwd(1197-1213) lac operator lac operator(8404-8420) lac o... lac operator(8404-8420) ...lac o lac operator(8404-8420) T7 promoter T7 promoter(1220-1238) T7 pr... T7 promoter(1220-1238) T7 pr... T7 promoter(1220-1238) SP6 promoter SP6 promoter(8344-8362) SP6 p... SP6 promoter(8344-8362) ...SP6 p SP6 promoter(8344-8362) CAP binding site CAP binding site(8473-8494) CAP b... CAP binding site(8473-8494) ...CAP b CAP binding site(8473-8494) lac promoter lac promoter(8428-8458) lac p... lac promoter(8428-8458) ...lac p lac promoter(8428-8458) AmpR promoter AmpR promoter(419-523) AmpR ... AmpR promoter(419-523) AmpR ... AmpR promoter(419-523)