pUC ori pUC ori(186-774) pUC o... pUC ori(186-774) CAP binding site CAP binding site(1062-1083) CAP b... CAP binding site(1062-1083) CAP b... CAP binding site(1062-1083) lac promoter lac promoter(1098-1128) lac p... lac promoter(1098-1128) lac p... lac promoter(1098-1128) lac operator lac operator(1136-1152) lac o... lac operator(1136-1152) lac o... lac operator(1136-1152) M13 rev M13 rev(1160-1176) M13 r... M13 rev(1160-1176) M13 r... M13 rev(1160-1176) T3 promoter T3 promoter(1197-1215) T3 pr... T3 promoter(1197-1215) T3 pr... T3 promoter(1197-1215) MNase MNase(1605-2045) MNase MNase(1605-2045) rrnB T1 terminator rrnB T1 terminator(2084-2170) rrnB ... rrnB T1 terminator(2084-2170) rrnB ... rrnB T1 terminator(2084-2170) rrnB T2 terminator rrnB T2 terminator(2189-2216) rrnB ... rrnB T2 terminator(2189-2216) rrnB ... rrnB T2 terminator(2189-2216) lacZα lacZα(6871-7028) lacZα lacZα(6871-7028) lacZα lacZα(6871-7028) AmpR promoter AmpR promoter(7365-7469) AmpR ... AmpR promoter(7365-7469) ...AmpR AmpR promoter(7365-7469) Ampicillin Ampicillin(7470-8330) Ampic... Ampicillin(7470-8330) M13 rev M13 rev(1162-1178) M13 r... M13 rev(1162-1178) M13 r... M13 rev(1162-1178) M13 fwd M13 fwd(6877-6893) M13 f... M13 fwd(6877-6893) ...M13 f M13 fwd(6877-6893) lac operator lac operator(1138-1154) lac o... lac operator(1138-1154) lac o... lac operator(1138-1154) T3 promoter T3 promoter(1199-1217) T3 pr... T3 promoter(1199-1217) T3 pr... T3 promoter(1199-1217) CAP binding site CAP binding site(1064-1085) CAP b... CAP binding site(1064-1085) CAP b... CAP binding site(1064-1085) rrnB T2 terminator rrnB T2 terminator(2191-2218) rrnB ... rrnB T2 terminator(2191-2218) rrnB ... rrnB T2 terminator(2191-2218) lac promoter lac promoter(1100-1130) lac p... lac promoter(1100-1130) lac p... lac promoter(1100-1130) rrnB T1 terminator rrnB T1 terminator(2086-2172) rrnB ... rrnB T1 terminator(2086-2172) rrnB ... rrnB T1 terminator(2086-2172) AmpR promoter AmpR promoter(7367-7471) AmpR ... AmpR promoter(7367-7471) ...AmpR AmpR promoter(7367-7471)