U6 promoter U6 promoter(1-241) U6 pr... U6 promoter(1-241) U6 pr... U6 promoter(1-241) MS2 stem loop MS2 stem loop(295-313) MS2 s... MS2 stem loop(295-313) MS2 s... MS2 stem loop(295-313) MS2 stem loop MS2 stem loop(365-383) MS2 s... MS2 stem loop(365-383) MS2 s... MS2 stem loop(365-383) PGK promoter PGK promoter(436-935) PGK p... PGK promoter(436-935) PuroR PuroR(975-1571) PuroR PuroR(975-1571) T2A T2A(1590-1643) T2A T2A(1590-1643) T2A T2A(1590-1643) TagBFP TagBFP(1644-2345) TagBF... TagBFP(1644-2345) Factor Xa site Factor Xa site(2823-2834) Facto... Factor Xa site(2823-2834) Facto... Factor Xa site(2823-2834) bGH poly(A) signal bGH poly(A) signal(3292-3516) bGH p... bGH poly(A) signal(3292-3516) bGH p... bGH poly(A) signal(3292-3516) T3 promoter T3 promoter(3542-3560) T3 pr... T3 promoter(3542-3560) T3 pr... T3 promoter(3542-3560) M13 rev M13 rev(3581-3597) M13 r... M13 rev(3581-3597) M13 r... M13 rev(3581-3597) lac operator lac operator(3605-3621) lac o... lac operator(3605-3621) lac o... lac operator(3605-3621) lac promoter lac promoter(3629-3659) lac p... lac promoter(3629-3659) lac p... lac promoter(3629-3659) CAP binding site CAP binding site(3674-3695) CAP b... CAP binding site(3674-3695) CAP b... CAP binding site(3674-3695) ori ori(3983-4571) ori ori(3983-4571) AmpR AmpR(4742-5602) AmpR AmpR(4742-5602) AmpR promoter AmpR promoter(5603-5707) AmpR ... AmpR promoter(5603-5707) ...AmpR AmpR promoter(5603-5707) f1 ori f1 ori(5733-6188) f1 or... f1 ori(5733-6188) M13 fwd M13 fwd(6330-6346) M13 f... M13 fwd(6330-6346) ...M13 f M13 fwd(6330-6346) T7 promoter T7 promoter(6356-6374) T7 pr... T7 promoter(6356-6374) ...T7 pr T7 promoter(6356-6374) CMV promoter CMV promoter(6690-6888) CMV p... CMV promoter(6690-6888) ...CMV p CMV promoter(6690-6888) 5' LTR (truncated) 5' LTR (truncated)(6906-7086) 5' LT... 5' LTR (truncated)(6906-7086) ...5' LT 5' LTR (truncated)(6906-7086) HIV-1 Ψ HIV-1 Ψ(7133-7258) HIV-1... HIV-1 Ψ(7133-7258) ...HIV-1 HIV-1 Ψ(7133-7258) RRE RRE(7757-7990) RRE RRE(7757-7990) gp41 peptide gp41 peptide(8175-8219) gp41 ... gp41 peptide(8175-8219) ...gp41 gp41 peptide(8175-8219) cPPT/CTS cPPT/CTS(8517-8634) cPPT/... cPPT/CTS(8517-8634) ...cPPT/ cPPT/CTS(8517-8634) Factor Xa site Factor Xa site(2825-2836) Facto... Factor Xa site(2825-2836) Facto... Factor Xa site(2825-2836) M13 rev M13 rev(3583-3599) M13 r... M13 rev(3583-3599) M13 r... M13 rev(3583-3599) M13 fwd M13 fwd(6332-6348) M13 f... M13 fwd(6332-6348) ...M13 f M13 fwd(6332-6348) lac operator lac operator(3607-3623) lac o... lac operator(3607-3623) lac o... lac operator(3607-3623) T7 promoter T7 promoter(6358-6376) T7 pr... T7 promoter(6358-6376) ...T7 pr T7 promoter(6358-6376) T3 promoter T3 promoter(3544-3562) T3 pr... T3 promoter(3544-3562) T3 pr... T3 promoter(3544-3562) CAP binding site CAP binding site(3676-3697) CAP b... CAP binding site(3676-3697) CAP b... CAP binding site(3676-3697) AmpR promoter AmpR promoter(5605-5709) AmpR ... AmpR promoter(5605-5709) ...AmpR AmpR promoter(5605-5709) cPPT/CTS cPPT/CTS(8519-8636) cPPT/... cPPT/CTS(8519-8636) ...cPPT/ cPPT/CTS(8519-8636) HIV-1 Ψ HIV-1 Ψ(7135-7260) HIV-1... HIV-1 Ψ(7135-7260) ...HIV-1 HIV-1 Ψ(7135-7260) RRE RRE(7759-7992) RRE RRE(7759-7992) U6 promoter U6 promoter(3-243) U6 pr... U6 promoter(3-243) U6 pr... U6 promoter(3-243) PGK promoter PGK promoter(438-937) PGK p... PGK promoter(438-937) PGK p... PGK promoter(438-937)