CMV promoter CMV promoter(1-199) CMV p... CMV promoter(1-199) CMV p... CMV promoter(1-199) 5' LTR (truncated) 5' LTR (truncated)(217-397) 5' LT... 5' LTR (truncated)(217-397) 5' LT... 5' LTR (truncated)(217-397) HIV-1 Ψ HIV-1 Ψ(444-569) HIV-1... HIV-1 Ψ(444-569) HIV-1... HIV-1 Ψ(444-569) RRE RRE(1062-1295) RRE RRE(1062-1295) RRE RRE(1062-1295) gp41 peptide gp41 peptide(1480-1524) gp41 ... gp41 peptide(1480-1524) gp41 ... gp41 peptide(1480-1524) cPPT/CTS cPPT/CTS(1822-1939) cPPT/... cPPT/CTS(1822-1939) cPPT/... cPPT/CTS(1822-1939) gRNA scaffold gRNA scaffold(2071-2146) gRNA ... gRNA scaffold(2071-2146) gRNA ... gRNA scaffold(2071-2146) EF-1α core promoter EF-1α core promoter(2208-2419) EF-1α... EF-1α core promoter(2208-2419) EF-1α... EF-1α core promoter(2208-2419) AsCpf1 AsCpf1(2444-6364) AsCpf... AsCpf1(2444-6364) Kozak sequence Kozak sequence(6084-6093) Kozak... Kozak sequence(6084-6093) Kozak... Kozak sequence(6084-6093) nucleoplasmin NLS nucleoplasmin NLS(6365-6412) nucle... nucleoplasmin NLS(6365-6412) nucle... nucleoplasmin NLS(6365-6412) FLAG FLAG(6413-6436) FLAG FLAG(6413-6436) FLAG FLAG(6413-6436) attB1 attB1(6479-6503) attB1 attB1(6479-6503) attB1 attB1(6479-6503) VP64 VP64(6533-6682) VP64 VP64(6533-6682) VP64 VP64(6533-6682) SV40 NLS SV40 NLS(6707-6727) SV40 ... SV40 NLS(6707-6727) SV40 ... SV40 NLS(6707-6727) RelA (p65) AD RelA (p65) AD(7157-7513) RelA ... RelA (p65) AD(7157-7513) ...RelA RelA (p65) AD(7157-7513) Rta AD Rta AD(7532-8101) Rta A... Rta AD(7532-8101) ...Rta A Rta AD(7532-8101) P2A P2A(8111-8167) P2A P2A(8111-8167) P2A P2A(8111-8167) PuroR PuroR(8168-8764) PuroR PuroR(8168-8764) WPRE WPRE(8780-9368) WPRE WPRE(8780-9368) Factor Xa site Factor Xa site(9251-9262) Facto... Factor Xa site(9251-9262) ...Facto Factor Xa site(9251-9262) 3' LTR (ΔU3) 3' LTR (ΔU3)(9440-9673) 3' LT... 3' LTR (ΔU3)(9440-9673) ...3' LT 3' LTR (ΔU3)(9440-9673) bGH poly(A) signal bGH poly(A) signal(9705-9929) bGH p... bGH poly(A) signal(9705-9929) ...bGH p bGH poly(A) signal(9705-9929) f1 ori f1 ori(9975-10403) f1 or... f1 ori(9975-10403) ...f1 or f1 ori(9975-10403) SV40 promoter SV40 promoter(10417-10746) SV40 ... SV40 promoter(10417-10746) ...SV40 SV40 promoter(10417-10746) SV40 ori SV40 ori(10597-10732) SV40 ... SV40 ori(10597-10732) ...SV40 SV40 ori(10597-10732) EM7 promoter EM7 promoter(10794-10841) EM7 p... EM7 promoter(10794-10841) ...EM7 p EM7 promoter(10794-10841) BleoR BleoR(10860-11234) BleoR BleoR(10860-11234) BleoR BleoR(10860-11234) SV40 poly(A) signal SV40 poly(A) signal(11364-11485) SV40 ... SV40 poly(A) signal(11364-11485) ...SV40 SV40 poly(A) signal(11364-11485) M13 rev M13 rev(11534-11550) M13 r... M13 rev(11534-11550) ...M13 r M13 rev(11534-11550) lac operator lac operator(11558-11574) lac o... lac operator(11558-11574) ...lac o lac operator(11558-11574) lac promoter lac promoter(11582-11612) lac p... lac promoter(11582-11612) ...lac p lac promoter(11582-11612) CAP binding site CAP binding site(11627-11648) CAP b... CAP binding site(11627-11648) ...CAP b CAP binding site(11627-11648) ori ori(11936-12524) ori ori(11936-12524) AmpR AmpR(12695-13555) AmpR AmpR(12695-13555) AmpR promoter AmpR promoter(13556-13660) AmpR ... AmpR promoter(13556-13660) ...AmpR AmpR promoter(13556-13660) CMV enhancer CMV enhancer(13926-14305) CMV e... CMV enhancer(13926-14305) ...CMV e CMV enhancer(13926-14305) Factor Xa site Factor Xa site(9253-9264) Facto... Factor Xa site(9253-9264) ...Facto Factor Xa site(9253-9264) M13 rev M13 rev(11536-11552) M13 r... M13 rev(11536-11552) ...M13 r M13 rev(11536-11552) lac operator lac operator(11560-11576) lac o... lac operator(11560-11576) ...lac o lac operator(11560-11576) attB1 attB1(6482-6502) attB1 attB1(6482-6502) attB1 attB1(6482-6502) CAP binding site CAP binding site(11629-11650) CAP b... CAP binding site(11629-11650) ...CAP b CAP binding site(11629-11650) lac promoter lac promoter(11584-11614) lac p... lac promoter(11584-11614) ...lac p lac promoter(11584-11614) EM7 promoter EM7 promoter(10796-10843) EM7 p... EM7 promoter(10796-10843) ...EM7 p EM7 promoter(10796-10843) gRNA scaffold gRNA scaffold(2073-2148) gRNA ... gRNA scaffold(2073-2148) gRNA ... gRNA scaffold(2073-2148) AmpR promoter AmpR promoter(13558-13662) AmpR ... AmpR promoter(13558-13662) ...AmpR AmpR promoter(13558-13662) cPPT/CTS cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) HIV-1 Ψ HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) bGH poly(A) signal bGH poly(A) signal(9707-9931) bGH p... bGH poly(A) signal(9707-9931) ...bGH p bGH poly(A) signal(9707-9931) RRE RRE(1064-1297) RRE RRE(1064-1297) RRE RRE(1064-1297) 3' LTR (ΔU3) 3' LTR (ΔU3)(9442-9675) 3' LT... 3' LTR (ΔU3)(9442-9675) ...3' LT 3' LTR (ΔU3)(9442-9675) BleoR BleoR(10862-11236) BleoR BleoR(10862-11236) BleoR BleoR(10862-11236) CMV enhancer CMV enhancer(13928-1) CMV e... CMV enhancer(13928-1) ...CMV e CMV enhancer(13928-1) WPRE WPRE(8782-9370) WPRE WPRE(8782-9370)