CMV promoter CMV promoter(1-199) CMV p... CMV promoter(1-199) CMV p... CMV promoter(1-199) 5' LTR (truncated) 5' LTR (truncated)(217-397) 5' LT... 5' LTR (truncated)(217-397) 5' LT... 5' LTR (truncated)(217-397) HIV-1 Ψ HIV-1 Ψ(444-569) HIV-1... HIV-1 Ψ(444-569) HIV-1... HIV-1 Ψ(444-569) RRE RRE(1062-1295) RRE RRE(1062-1295) RRE RRE(1062-1295) gp41 peptide gp41 peptide(1480-1524) gp41 ... gp41 peptide(1480-1524) gp41 ... gp41 peptide(1480-1524) cPPT/CTS cPPT/CTS(1822-1939) cPPT/... cPPT/CTS(1822-1939) cPPT/... cPPT/CTS(1822-1939) gRNA scaffold gRNA scaffold(2071-2146) gRNA ... gRNA scaffold(2071-2146) gRNA ... gRNA scaffold(2071-2146) EF-1α core promoter EF-1α core promoter(2208-2419) EF-1α... EF-1α core promoter(2208-2419) EF-1α... EF-1α core promoter(2208-2419) nucleoplasmin NLS nucleoplasmin NLS(5603-5650) nucle... nucleoplasmin NLS(5603-5650) nucle... nucleoplasmin NLS(5603-5650) FLAG FLAG(5651-5674) FLAG FLAG(5651-5674) FLAG FLAG(5651-5674) attB1 attB1(5717-5741) attB1 attB1(5717-5741) attB1 attB1(5717-5741) VP64 VP64(5771-5920) VP64 VP64(5771-5920) VP64 VP64(5771-5920) SV40 NLS SV40 NLS(5945-5965) SV40 ... SV40 NLS(5945-5965) SV40 ... SV40 NLS(5945-5965) RelA (p65) AD RelA (p65) AD(6395-6751) RelA ... RelA (p65) AD(6395-6751) RelA ... RelA (p65) AD(6395-6751) Rta AD Rta AD(6770-7339) Rta A... Rta AD(6770-7339) ...Rta A Rta AD(6770-7339) P2A P2A(7349-7405) P2A P2A(7349-7405) P2A P2A(7349-7405) PuroR PuroR(7406-8002) PuroR PuroR(7406-8002) WPRE WPRE(8018-8606) WPRE WPRE(8018-8606) Factor Xa site Factor Xa site(8489-8500) Facto... Factor Xa site(8489-8500) ...Facto Factor Xa site(8489-8500) 3' LTR (ΔU3) 3' LTR (ΔU3)(8678-8911) 3' LT... 3' LTR (ΔU3)(8678-8911) ...3' LT 3' LTR (ΔU3)(8678-8911) bGH poly(A) signal bGH poly(A) signal(8943-9167) bGH p... bGH poly(A) signal(8943-9167) ...bGH p bGH poly(A) signal(8943-9167) f1 ori f1 ori(9213-9641) f1 or... f1 ori(9213-9641) ...f1 or f1 ori(9213-9641) SV40 promoter SV40 promoter(9655-9984) SV40 ... SV40 promoter(9655-9984) ...SV40 SV40 promoter(9655-9984) SV40 ori SV40 ori(9835-9970) SV40 ... SV40 ori(9835-9970) ...SV40 SV40 ori(9835-9970) EM7 promoter EM7 promoter(10032-10079) EM7 p... EM7 promoter(10032-10079) ...EM7 p EM7 promoter(10032-10079) BleoR BleoR(10098-10472) BleoR BleoR(10098-10472) BleoR BleoR(10098-10472) SV40 poly(A) signal SV40 poly(A) signal(10602-10723) SV40 ... SV40 poly(A) signal(10602-10723) ...SV40 SV40 poly(A) signal(10602-10723) M13 rev M13 rev(10772-10788) M13 r... M13 rev(10772-10788) ...M13 r M13 rev(10772-10788) lac operator lac operator(10796-10812) lac o... lac operator(10796-10812) ...lac o lac operator(10796-10812) lac promoter lac promoter(10820-10850) lac p... lac promoter(10820-10850) ...lac p lac promoter(10820-10850) CAP binding site CAP binding site(10865-10886) CAP b... CAP binding site(10865-10886) ...CAP b CAP binding site(10865-10886) ori ori(11174-11762) ori ori(11174-11762) AmpR AmpR(11933-12793) AmpR AmpR(11933-12793) AmpR promoter AmpR promoter(12794-12898) AmpR ... AmpR promoter(12794-12898) ...AmpR AmpR promoter(12794-12898) CMV enhancer CMV enhancer(13164-13543) CMV e... CMV enhancer(13164-13543) ...CMV e CMV enhancer(13164-13543) Factor Xa site Factor Xa site(8491-8502) Facto... Factor Xa site(8491-8502) ...Facto Factor Xa site(8491-8502) M13 rev M13 rev(10774-10790) M13 r... M13 rev(10774-10790) ...M13 r M13 rev(10774-10790) lac operator lac operator(10798-10814) lac o... lac operator(10798-10814) ...lac o lac operator(10798-10814) attB1 attB1(5720-5740) attB1 attB1(5720-5740) attB1 attB1(5720-5740) CAP binding site CAP binding site(10867-10888) CAP b... CAP binding site(10867-10888) ...CAP b CAP binding site(10867-10888) lac promoter lac promoter(10822-10852) lac p... lac promoter(10822-10852) ...lac p lac promoter(10822-10852) nucleoplasmin NLS nucleoplasmin NLS(5605-5652) nucle... nucleoplasmin NLS(5605-5652) nucle... nucleoplasmin NLS(5605-5652) EM7 promoter EM7 promoter(10034-10081) EM7 p... EM7 promoter(10034-10081) ...EM7 p EM7 promoter(10034-10081) gRNA scaffold gRNA scaffold(2073-2148) gRNA ... gRNA scaffold(2073-2148) gRNA ... gRNA scaffold(2073-2148) AmpR promoter AmpR promoter(12796-12900) AmpR ... AmpR promoter(12796-12900) ...AmpR AmpR promoter(12796-12900) cPPT/CTS cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) cPPT/... cPPT/CTS(1824-1941) HIV-1 Ψ HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) HIV-1... HIV-1 Ψ(446-571) bGH poly(A) signal bGH poly(A) signal(8945-9169) bGH p... bGH poly(A) signal(8945-9169) ...bGH p bGH poly(A) signal(8945-9169) RRE RRE(1064-1297) RRE RRE(1064-1297) RRE RRE(1064-1297) 3' LTR (ΔU3) 3' LTR (ΔU3)(8680-8913) 3' LT... 3' LTR (ΔU3)(8680-8913) ...3' LT 3' LTR (ΔU3)(8680-8913) BleoR BleoR(10100-10474) BleoR BleoR(10100-10474) BleoR BleoR(10100-10474) CMV enhancer CMV enhancer(13166-1) CMV e... CMV enhancer(13166-1) ...CMV e CMV enhancer(13166-1) WPRE WPRE(8020-8608) WPRE WPRE(8020-8608)