3xFLAG 3xFLAG(463-528) 3xFLA... 3xFLAG(463-528) 3xFLA... 3xFLAG(463-528) SV40 NLS SV40 NLS(535-555) SV40 ... SV40 NLS(535-555) SV40 ... SV40 NLS(535-555) Factor Xa site Factor Xa site(3152-3163) Facto... Factor Xa site(3152-3163) Facto... Factor Xa site(3152-3163) SV40 NLS SV40 NLS(4681-4701) SV40 ... SV40 NLS(4681-4701) SV40 ... SV40 NLS(4681-4701) SV40 NLS SV40 NLS(4996-5016) SV40 ... SV40 NLS(4996-5016) SV40 ... SV40 NLS(4996-5016) PuroR PuroR(5077-5676) PuroR PuroR(5077-5676) loxP loxP(5730-5763) loxP loxP(5730-5763) loxP loxP(5730-5763) WPRE WPRE(5788-6376) WPRE WPRE(5788-6376) Factor Xa site Factor Xa site(6259-6270) Facto... Factor Xa site(6259-6270) Facto... Factor Xa site(6259-6270) KS primer KS primer(6379-6395) KS pr... KS primer(6379-6395) KS pr... KS primer(6379-6395) 5' LTR 5' LTR(6901-7081) 5' LT... 5' LTR(6901-7081) 5' LT... 5' LTR(6901-7081) bGH poly(A) signal bGH poly(A) signal(7113-7337) bGH p... bGH poly(A) signal(7113-7337) bGH p... bGH poly(A) signal(7113-7337) f1 ori f1 ori(7383-7811) f1 or... f1 ori(7383-7811) ...f1 or f1 ori(7383-7811) SV40 promoter SV40 promoter(7825-8154) SV40 ... SV40 promoter(7825-8154) ...SV40 SV40 promoter(7825-8154) SV40 ori SV40 ori(8005-8140) SV40 ... SV40 ori(8005-8140) ...SV40 SV40 ori(8005-8140) EM7 promoter EM7 promoter(8202-8249) EM7 p... EM7 promoter(8202-8249) ...EM7 p EM7 promoter(8202-8249) BleoR BleoR(8268-8642) BleoR BleoR(8268-8642) BleoR BleoR(8268-8642) SV40 poly(A) signal SV40 poly(A) signal(8772-8893) SV40 ... SV40 poly(A) signal(8772-8893) ...SV40 SV40 poly(A) signal(8772-8893) M13 rev M13 rev(8942-8958) M13 r... M13 rev(8942-8958) ...M13 r M13 rev(8942-8958) lac operator lac operator(8966-8982) lac o... lac operator(8966-8982) ...lac o lac operator(8966-8982) lac promoter lac promoter(8990-9020) lac p... lac promoter(8990-9020) ...lac p lac promoter(8990-9020) ori ori(9344-9932) ori ori(9344-9932) AmpR AmpR(10103-10963) AmpR AmpR(10103-10963) AmpR promoter AmpR promoter(10964-11068) AmpR ... AmpR promoter(10964-11068) ...AmpR AmpR promoter(10964-11068) CMV enhancer CMV enhancer(11334-11713) CMV e... CMV enhancer(11334-11713) ...CMV e CMV enhancer(11334-11713) 5' LTR 5' LTR(11931-12111) 5' LT... 5' LTR(11931-12111) ...5' LT 5' LTR(11931-12111) HIV-1 ψ HIV-1 ψ(12158-12283) HIV-1... HIV-1 ψ(12158-12283) ...HIV-1 HIV-1 ψ(12158-12283) HIV-1 RRE HIV-1 RRE(12776-13009) HIV-1... HIV-1 RRE(12776-13009) ...HIV-1 HIV-1 RRE(12776-13009) HIV-1 cPPT HIV-1 cPPT(13536-13653) HIV-1... HIV-1 cPPT(13536-13653) ...HIV-1 HIV-1 cPPT(13536-13653) loxP loxP(13697-13730) loxP loxP(13697-13730) loxP loxP(13697-13730) Factor Xa site Factor Xa site(6261-6272) Facto... Factor Xa site(6261-6272) Facto... Factor Xa site(6261-6272) enterokinase site enterokinase site(516-530) enter... enterokinase site(516-530) enter... enterokinase site(516-530) KS primer KS primer(6381-6397) KS pr... KS primer(6381-6397) KS pr... KS primer(6381-6397) M13 rev M13 rev(8944-8960) M13 r... M13 rev(8944-8960) ...M13 r M13 rev(8944-8960) lac operator lac operator(8968-8984) lac o... lac operator(8968-8984) ...lac o lac operator(8968-8984) CAP binding site CAP binding site(9037-9058) CAP b... CAP binding site(9037-9058) ...CAP b CAP binding site(9037-9058) lac promoter lac promoter(8992-9022) lac p... lac promoter(8992-9022) ...lac p lac promoter(8992-9022) loxP loxP(5732-5765) loxP loxP(5732-5765) loxP loxP(5732-5765) loxP loxP(13699-13732) loxP loxP(13699-13732) loxP loxP(13699-13732) EM7 promoter EM7 promoter(8204-8251) EM7 p... EM7 promoter(8204-8251) ...EM7 p EM7 promoter(8204-8251) gRNA scaffold gRNA scaffold(13743-13818) gRNA ... gRNA scaffold(13743-13818) ...gRNA gRNA scaffold(13743-13818) AmpR promoter AmpR promoter(10966-11070) AmpR ... AmpR promoter(10966-11070) ...AmpR AmpR promoter(10966-11070) cPPT/CTS cPPT/CTS(13538-13655) cPPT/... cPPT/CTS(13538-13655) ...cPPT/ cPPT/CTS(13538-13655) HIV-1 Ψ HIV-1 Ψ(12160-12285) HIV-1... HIV-1 Ψ(12160-12285) ...HIV-1 HIV-1 Ψ(12160-12285) bGH poly(A) signal bGH poly(A) signal(7115-7339) bGH p... bGH poly(A) signal(7115-7339) bGH p... bGH poly(A) signal(7115-7339) RRE RRE(12778-13011) RRE RRE(12778-13011) RRE RRE(12778-13011) BleoR BleoR(8270-8644) BleoR BleoR(8270-8644) BleoR BleoR(8270-8644) CMV enhancer CMV enhancer(11336-11715) CMV e... CMV enhancer(11336-11715) ...CMV e CMV enhancer(11336-11715) WPRE WPRE(5790-6378) WPRE WPRE(5790-6378)