ori ori(514-1102) ori ori(514-1102) AmpR AmpR(1273-2133) AmpR AmpR(1273-2133) AmpR promoter AmpR promoter(2134-2238) AmpR ... AmpR promoter(2134-2238) AmpR ... AmpR promoter(2134-2238) SP6 promoter SP6 promoter(3026-3044) SP6 p... SP6 promoter(3026-3044) SP6 p... SP6 promoter(3026-3044) 3' LTR (ΔU3) 3' LTR (ΔU3)(3069-3302) 3' LT... 3' LTR (ΔU3)(3069-3302) 3' LT... 3' LTR (ΔU3)(3069-3302) KS primer KS primer(3393-3409) KS pr... KS primer(3393-3409) KS pr... KS primer(3393-3409) Factor Xa site Factor Xa site(3518-3529) Facto... Factor Xa site(3518-3529) Facto... Factor Xa site(3518-3529) WPRE WPRE(3412-4000) WPRE WPRE(3412-4000) KRAB KRAB(4064-4249) KRAB KRAB(4064-4249) KRAB KRAB(4064-4249) TagBFP TagBFP(4295-4990) TagBF... TagBFP(4295-4990) TagBF... TagBFP(4295-4990) SV40 NLS SV40 NLS(5030-5050) SV40 ... SV40 NLS(5030-5050) SV40 ... SV40 NLS(5030-5050) SV40 NLS SV40 NLS(5057-5077) SV40 ... SV40 NLS(5057-5077) SV40 ... SV40 NLS(5057-5077) HA HA(5090-5116) HA HA(5090-5116) HA HA(5090-5116) HA HA(5096-5122) HA HA(5096-5122) HA HA(5096-5122) SV40 ori SV40 ori(14120-88) SV40 ... SV40 ori(14120-88) SV40 ... SV40 ori(14120-88) dCas9 dCas9(5126-9229) dCas9 dCas9(5126-9229) Cas9 Cas9(5126-9229) Cas9 Cas9(5126-9229) SV40 promoter SV40 promoter(14106-268) SV40 ... SV40 promoter(14106-268) SV40 ... SV40 promoter(14106-268) Kozak sequence Kozak sequence(9226-9235) Kozak... Kozak sequence(9226-9235) ...Kozak Kozak sequence(9226-9235) SFFV promoter SFFV promoter(9304-9711) SFFV ... SFFV promoter(9304-9711) ...SFFV SFFV promoter(9304-9711) cPPT/CTS cPPT/CTS(9864-9981) cPPT/... cPPT/CTS(9864-9981) ...cPPT/ cPPT/CTS(9864-9981) gp41 peptide gp41 peptide(10266-10310) gp41 ... gp41 peptide(10266-10310) ...gp41 gp41 peptide(10266-10310) RRE RRE(10495-10728) RRE RRE(10495-10728) RRE RRE(10495-10728) HIV-1 Ψ HIV-1 Ψ(11225-11350) HIV-1... HIV-1 Ψ(11225-11350) ...HIV-1 HIV-1 Ψ(11225-11350) 3' LTR 3' LTR(11397-12030) 3' LT... 3' LTR(11397-12030) ...3' LT 3' LTR(11397-12030) SV40 poly(A) signal SV40 poly(A) signal(12198-12332) SV40 ... SV40 poly(A) signal(12198-12332) ...SV40 SV40 poly(A) signal(12198-12332) SV40 NLS SV40 NLS(12757-12777) SV40 ... SV40 NLS(12757-12777) ...SV40 SV40 NLS(12757-12777) small t intron small t intron(12907-12972) small... small t intron(12907-12972) ...small small t intron(12907-12972) Factor Xa site Factor Xa site(3520-3531) Facto... Factor Xa site(3520-3531) Facto... Factor Xa site(3520-3531) KS primer KS primer(3395-3411) KS pr... KS primer(3395-3411) KS pr... KS primer(3395-3411) SP6 promoter SP6 promoter(3028-3046) SP6 p... SP6 promoter(3028-3046) SP6 p... SP6 promoter(3028-3046) SV40 NLS SV40 NLS(12759-12779) SV40 ... SV40 NLS(12759-12779) ...SV40 SV40 NLS(12759-12779) small t intron small t intron(12909-12974) small... small t intron(12909-12974) ...small small t intron(12909-12974) AmpR promoter AmpR promoter(2136-2240) AmpR ... AmpR promoter(2136-2240) AmpR ... AmpR promoter(2136-2240) cPPT/CTS cPPT/CTS(9866-9983) cPPT/... cPPT/CTS(9866-9983) ...cPPT/ cPPT/CTS(9866-9983) HIV-1 Ψ HIV-1 Ψ(11227-11352) HIV-1... HIV-1 Ψ(11227-11352) ...HIV-1 HIV-1 Ψ(11227-11352) SV40 poly(A) signal SV40 poly(A) signal(12200-12334) SV40 ... SV40 poly(A) signal(12200-12334) ...SV40 SV40 poly(A) signal(12200-12334) RRE RRE(10497-10730) RRE RRE(10497-10730) RRE RRE(10497-10730) 3' LTR (ΔU3) 3' LTR (ΔU3)(3071-3304) 3' LT... 3' LTR (ΔU3)(3071-3304) 3' LT... 3' LTR (ΔU3)(3071-3304) WPRE WPRE(3414-4002) WPRE WPRE(3414-4002)