ori ori(463-1051) ori ori(463-1051) AmpR AmpR(1222-2082) AmpR AmpR(1222-2082) AmpR promoter AmpR promoter(2083-2187) AmpR ... AmpR promoter(2083-2187) AmpR ... AmpR promoter(2083-2187) SP6 promoter SP6 promoter(2975-2993) SP6 p... SP6 promoter(2975-2993) SP6 p... SP6 promoter(2975-2993) 3' LTR (ΔU3) 3' LTR (ΔU3)(3018-3251) 3' LT... 3' LTR (ΔU3)(3018-3251) 3' LT... 3' LTR (ΔU3)(3018-3251) KS primer KS primer(3342-3358) KS pr... KS primer(3342-3358) KS pr... KS primer(3342-3358) Factor Xa site Factor Xa site(3467-3478) Facto... Factor Xa site(3467-3478) Facto... Factor Xa site(3467-3478) WPRE WPRE(3361-3949) WPRE WPRE(3361-3949) mCherry mCherry(4009-4719) mCher... mCherry(4009-4719) P2A P2A(4720-4776) P2A P2A(4720-4776) P2A P2A(4720-4776) SV40 NLS SV40 NLS(4816-4836) SV40 ... SV40 NLS(4816-4836) SV40 ... SV40 NLS(4816-4836) SV40 NLS SV40 NLS(4843-4863) SV40 ... SV40 NLS(4843-4863) SV40 ... SV40 NLS(4843-4863) HA HA(4876-4902) HA HA(4876-4902) HA HA(4876-4902) HA HA(4882-4908) HA HA(4882-4908) HA HA(4882-4908) dCas9 dCas9(4912-9015) dCas9 dCas9(4912-9015) Cas9 Cas9(4912-9015) Cas9 Cas9(4912-9015) SV40 ori SV40 ori(14149-37) SV40 ... SV40 ori(14149-37) ...SV40 SV40 ori(14149-37) SV40 promoter SV40 promoter(14135-217) SV40 ... SV40 promoter(14135-217) SV40 ... SV40 promoter(14135-217) KRAB KRAB(9037-9222) KRAB KRAB(9037-9222) KRAB KRAB(9037-9222) Kozak sequence Kozak sequence(9249-9258) Kozak... Kozak sequence(9249-9258) ...Kozak Kozak sequence(9249-9258) SFFV promoter SFFV promoter(9333-9740) SFFV ... SFFV promoter(9333-9740) ...SFFV SFFV promoter(9333-9740) cPPT/CTS cPPT/CTS(9893-10010) cPPT/... cPPT/CTS(9893-10010) ...cPPT/ cPPT/CTS(9893-10010) gp41 peptide gp41 peptide(10295-10339) gp41 ... gp41 peptide(10295-10339) ...gp41 gp41 peptide(10295-10339) RRE RRE(10524-10757) RRE RRE(10524-10757) RRE RRE(10524-10757) HIV-1 Ψ HIV-1 Ψ(11254-11379) HIV-1... HIV-1 Ψ(11254-11379) ...HIV-1 HIV-1 Ψ(11254-11379) 3' LTR 3' LTR(11426-12059) 3' LT... 3' LTR(11426-12059) ...3' LT 3' LTR(11426-12059) SV40 poly(A) signal SV40 poly(A) signal(12227-12361) SV40 ... SV40 poly(A) signal(12227-12361) ...SV40 SV40 poly(A) signal(12227-12361) SV40 NLS SV40 NLS(12786-12806) SV40 ... SV40 NLS(12786-12806) ...SV40 SV40 NLS(12786-12806) small t intron small t intron(12936-13001) small... small t intron(12936-13001) ...small small t intron(12936-13001) Factor Xa site Factor Xa site(3469-3480) Facto... Factor Xa site(3469-3480) Facto... Factor Xa site(3469-3480) KS primer KS primer(3344-3360) KS pr... KS primer(3344-3360) KS pr... KS primer(3344-3360) SP6 promoter SP6 promoter(2977-2995) SP6 p... SP6 promoter(2977-2995) SP6 p... SP6 promoter(2977-2995) SV40 NLS SV40 NLS(12788-12808) SV40 ... SV40 NLS(12788-12808) ...SV40 SV40 NLS(12788-12808) small t intron small t intron(12938-13003) small... small t intron(12938-13003) ...small small t intron(12938-13003) AmpR promoter AmpR promoter(2085-2189) AmpR ... AmpR promoter(2085-2189) AmpR ... AmpR promoter(2085-2189) cPPT/CTS cPPT/CTS(9895-10012) cPPT/... cPPT/CTS(9895-10012) ...cPPT/ cPPT/CTS(9895-10012) HIV-1 Ψ HIV-1 Ψ(11256-11381) HIV-1... HIV-1 Ψ(11256-11381) ...HIV-1 HIV-1 Ψ(11256-11381) SV40 poly(A) signal SV40 poly(A) signal(12229-12363) SV40 ... SV40 poly(A) signal(12229-12363) ...SV40 SV40 poly(A) signal(12229-12363) RRE RRE(10526-10759) RRE RRE(10526-10759) RRE RRE(10526-10759) 3' LTR (ΔU3) 3' LTR (ΔU3)(3020-3253) 3' LT... 3' LTR (ΔU3)(3020-3253) 3' LT... 3' LTR (ΔU3)(3020-3253) WPRE WPRE(3363-3951) WPRE WPRE(3363-3951)