5' LTR 5' LTR(733-1249) 5' LT... 5' LTR(733-1249) MESV ψ MESV ψ(1313-2137) MESV ... MESV ψ(1313-2137) KS primer KS primer(2156-2172) KS pr... KS primer(2156-2172) KS pr... KS primer(2156-2172) U6 promoter U6 promoter(2246-2486) U6 pr... U6 promoter(2246-2486) U6 pr... U6 promoter(2246-2486) MMLV 5' LTR-ΔU3 MMLV 5' LTR-ΔU3(3953-4127) MMLV ... MMLV 5' LTR-ΔU3(3953-4127) ...MMLV MMLV 5' LTR-ΔU3(3953-4127) lac operator lac operator(4289-4305) lac o... lac operator(4289-4305) ...lac o lac operator(4289-4305) lac promoter lac promoter(4313-4343) lac p... lac promoter(4313-4343) ...lac p lac promoter(4313-4343) CAP binding site CAP binding site(4358-4379) CAP b... CAP binding site(4358-4379) ...CAP b CAP binding site(4358-4379) pUC ori pUC ori(4667-5255) pUC o... pUC ori(4667-5255) Ampicillin Ampicillin(5426-6286) Ampic... Ampicillin(5426-6286) AmpR promoter AmpR promoter(6287-6391) AmpR ... AmpR promoter(6287-6391) ...AmpR AmpR promoter(6287-6391) KS primer KS primer(2158-2174) KS pr... KS primer(2158-2174) KS pr... KS primer(2158-2174) lac operator lac operator(4291-4307) lac o... lac operator(4291-4307) ...lac o lac operator(4291-4307) CAP binding site CAP binding site(4360-4381) CAP b... CAP binding site(4360-4381) ...CAP b CAP binding site(4360-4381) lac promoter lac promoter(4315-4345) lac p... lac promoter(4315-4345) ...lac p lac promoter(4315-4345) AmpR promoter AmpR promoter(6289-6393) AmpR ... AmpR promoter(6289-6393) ...AmpR AmpR promoter(6289-6393) MESV Ψ MESV Ψ(1315-1656) MESV ... MESV Ψ(1315-1656) MESV ... MESV Ψ(1315-1656) gag (truncated) gag (truncated)(1723-2139) gag (... gag (truncated)(1723-2139) PGK promoter PGK promoter(2582-3081) PGK p... PGK promoter(2582-3081) 5' LTR 5' LTR(735-1251) 5' LT... 5' LTR(735-1251)