MESV ψ MESV ψ(579-1403) MESV ... MESV ψ(579-1403) KS primer KS primer(1422-1438) KS pr... KS primer(1422-1438) KS pr... KS primer(1422-1438) T7 promoter T7 promoter(1560-1578) T7 pr... T7 promoter(1560-1578) T7 pr... T7 promoter(1560-1578) CMV CMV(1622-2209) CMV CMV(1622-2209) H1 promoter H1 promoter(2430-2644) H1 pr... H1 promoter(2430-2644) H1 pr... H1 promoter(2430-2644) mPGK mPGK(2648-3162) mPGK mPGK(2648-3162) Kozak Kozak(3187-3192) Kozak Kozak(3187-3192) Kozak Kozak(3187-3192) EGFP EGFP(3193-3910) EGFP EGFP(3193-3910) Kozak Kozak(3967-3972) Kozak Kozak(3967-3972) Kozak Kozak(3967-3972) Neo Neo(3973-4767) Neo Neo(3973-4767) 5' LTR 5' LTR(8370-515) 5' LT... 5' LTR(8370-515) poly(A) signal poly(A) signal(4831-4879) poly(... poly(A) signal(4831-4879) ...poly( poly(A) signal(4831-4879) MMLV 5' LTR-ΔU3 MMLV 5' LTR-ΔU3(5104-5278) MMLV ... MMLV 5' LTR-ΔU3(5104-5278) ...MMLV MMLV 5' LTR-ΔU3(5104-5278) lac operator lac operator(5440-5456) lac o... lac operator(5440-5456) ...lac o lac operator(5440-5456) lac promoter lac promoter(5464-5494) lac p... lac promoter(5464-5494) ...lac p lac promoter(5464-5494) CAP binding site CAP binding site(5509-5530) CAP b... CAP binding site(5509-5530) ...CAP b CAP binding site(5509-5530) pUC ori pUC ori(5818-6406) pUC o... pUC ori(5818-6406) Ampicillin Ampicillin(6577-7437) Ampic... Ampicillin(6577-7437) AmpR promoter AmpR promoter(7438-7542) AmpR ... AmpR promoter(7438-7542) ...AmpR AmpR promoter(7438-7542) KS primer KS primer(1424-1440) KS pr... KS primer(1424-1440) KS pr... KS primer(1424-1440) lac operator lac operator(5442-5458) lac o... lac operator(5442-5458) ...lac o lac operator(5442-5458) T7 promoter T7 promoter(1562-1580) T7 pr... T7 promoter(1562-1580) T7 pr... T7 promoter(1562-1580) CAP binding site CAP binding site(5511-5532) CAP b... CAP binding site(5511-5532) ...CAP b CAP binding site(5511-5532) lac promoter lac promoter(5466-5496) lac p... lac promoter(5466-5496) ...lac p lac promoter(5466-5496) poly(A) signal poly(A) signal(4833-4881) poly(... poly(A) signal(4833-4881) ...poly( poly(A) signal(4833-4881) AmpR promoter AmpR promoter(7440-7544) AmpR ... AmpR promoter(7440-7544) ...AmpR AmpR promoter(7440-7544) CMV promoter CMV promoter(1625-1828) CMV p... CMV promoter(1625-1828) CMV p... CMV promoter(1625-1828) MESV Ψ MESV Ψ(581-922) MESV ... MESV Ψ(581-922) MESV ... MESV Ψ(581-922) CMV enhancer CMV enhancer(1829-2208) CMV e... CMV enhancer(1829-2208) CMV e... CMV enhancer(1829-2208) gag (truncated) gag (truncated)(989-1405) gag (... gag (truncated)(989-1405) gag (... gag (truncated)(989-1405)