3' LTR 3' LTR(2-635) 3' LT... 3' LTR(2-635) HIV-1 Ψ HIV-1 Ψ(682-807) HIV-1... HIV-1 Ψ(682-807) HIV-1... HIV-1 Ψ(682-807) RRE RRE(1300-1533) RRE RRE(1300-1533) EF-1α promoter EF-1α promoter(2122-3299) EF-1α... EF-1α promoter(2122-3299) cPPTCTS cPPTCTS(3347-3464) cPPTC... cPPTCTS(3347-3464) cPPTC... cPPTCTS(3347-3464) Kozak Kozak(3548-3553) Kozak Kozak(3548-3553) Kozak Kozak(3548-3553) EGFP EGFP(3554-4271) EGFP EGFP(3554-4271) WPRE WPRE(4343-4931) WPRE WPRE(4343-4931) tetracycline response element tetracycline response element(5099-5369) tetra... tetracycline response element(5099-5369) tetra... tetracycline response element(5099-5369) H1 promoter H1 promoter(5398-5612) H1 pr... H1 promoter(5398-5612) H1 pr... H1 promoter(5398-5612) loxP loxP(5647-5680) loxP loxP(5647-5680) loxP loxP(5647-5680) SP6 promoter SP6 promoter(5904-5923) SP6 p... SP6 promoter(5904-5923) ...SP6 p SP6 promoter(5904-5923) AmpR promoter AmpR promoter(6711-6815) AmpR ... AmpR promoter(6711-6815) ...AmpR AmpR promoter(6711-6815) Ampicillin Ampicillin(6816-7676) Ampic... Ampicillin(6816-7676) pBR322 ori pBR322 ori(7847-8435) pBR32... pBR322 ori(7847-8435) SV40 promoter SV40 promoter(8681-9010) SV40 ... SV40 promoter(8681-9010) ...SV40 SV40 promoter(8681-9010) small t intron small t intron(10144-10209) small... small t intron(10144-10209) ...small small t intron(10144-10209) SV40-NLS SV40-NLS(10339-10359) SV40-... SV40-NLS(10339-10359) ...SV40- SV40-NLS(10339-10359) SV40 early pA SV40 early pA(10678-10918) SV40 ... SV40 early pA(10678-10918) ...SV40 SV40 early pA(10678-10918) Factor Xa site Factor Xa site(4816-4827) Facto... Factor Xa site(4816-4827) Facto... Factor Xa site(4816-4827) tet operator tet operator(5101-5119) tet o... tet operator(5101-5119) tet o... tet operator(5101-5119) tet operator tet operator(5143-5161) tet o... tet operator(5143-5161) tet o... tet operator(5143-5161) tet operator tet operator(5185-5203) tet o... tet operator(5185-5203) tet o... tet operator(5185-5203) tet operator tet operator(5227-5245) tet o... tet operator(5227-5245) tet o... tet operator(5227-5245) tet operator tet operator(5269-5287) tet o... tet operator(5269-5287) tet o... tet operator(5269-5287) tet operator tet operator(5311-5329) tet o... tet operator(5311-5329) tet o... tet operator(5311-5329) tet operator tet operator(5353-5371) tet o... tet operator(5353-5371) tet o... tet operator(5353-5371) SP6 promoter SP6 promoter(5907-5925) SP6 p... SP6 promoter(5907-5925) ...SP6 p SP6 promoter(5907-5925) SV40 NLS SV40 NLS(10341-10361) SV40 ... SV40 NLS(10341-10361) ...SV40 SV40 NLS(10341-10361) loxP loxP(5649-5682) loxP loxP(5649-5682) loxP loxP(5649-5682) small t intron small t intron(10146-10211) small... small t intron(10146-10211) ...small small t intron(10146-10211) AmpR promoter AmpR promoter(6713-6817) AmpR ... AmpR promoter(6713-6817) ...AmpR AmpR promoter(6713-6817) cPPT/CTS cPPT/CTS(3349-3466) cPPT/... cPPT/CTS(3349-3466) cPPT/... cPPT/CTS(3349-3466) HIV-1 Ψ HIV-1 Ψ(684-809) HIV-1... HIV-1 Ψ(684-809) HIV-1... HIV-1 Ψ(684-809) SV40 poly(A) signal SV40 poly(A) signal(10786-10920) SV40 ... SV40 poly(A) signal(10786-10920) ...SV40 SV40 poly(A) signal(10786-10920) SV40 ori SV40 ori(8863-8998) SV40 ... SV40 ori(8863-8998) ...SV40 SV40 ori(8863-8998) RRE RRE(1302-1535) RRE RRE(1302-1535) RRE RRE(1302-1535) tetracycline response element tetracycline response element(5101-5371) tetra... tetracycline response element(5101-5371) tetra... tetracycline response element(5101-5371) WPRE WPRE(4345-4933) WPRE WPRE(4345-4933)