M13 fwd M13 fwd(379-395) M13 f... M13 fwd(379-395) M13 f... M13 fwd(379-395) M13 rev M13 rev(553-569) M13 r... M13 rev(553-569) M13 r... M13 rev(553-569) lac operator lac operator(577-593) lac o... lac operator(577-593) lac o... lac operator(577-593) lac promoter lac promoter(601-631) lac p... lac promoter(601-631) lac p... lac promoter(601-631) ori ori(942-1530) ori ori(942-1530) KanR KanR(1701-2516) KanR KanR(1701-2516) AmpR promoter AmpR promoter(2517-2621) AmpR ... AmpR promoter(2517-2621) ...AmpR AmpR promoter(2517-2621) M13 rev M13 rev(555-571) M13 r... M13 rev(555-571) M13 r... M13 rev(555-571) M13 fwd M13 fwd(381-397) M13 f... M13 fwd(381-397) M13 f... M13 fwd(381-397) lac operator lac operator(579-595) lac o... lac operator(579-595) lac o... lac operator(579-595) CAP binding site CAP binding site(648-669) CAP b... CAP binding site(648-669) CAP b... CAP binding site(648-669) lac promoter lac promoter(603-633) lac p... lac promoter(603-633) lac p... lac promoter(603-633) AmpR promoter AmpR promoter(2519-2623) AmpR ... AmpR promoter(2519-2623) ...AmpR AmpR promoter(2519-2623)