CMV enhancer CMV enhancer(59-364) CMV e... CMV enhancer(59-364) CMV promoter CMV promoter(365-568) CMV p... CMV promoter(365-568) CMV p... CMV promoter(365-568) MCS MCS(609-665) MCS MCS(609-665) MCS MCS(609-665) IRES IRES(667-1240) IRES IRES(667-1240) EGFP EGFP(1254-1973) EGFP EGFP(1254-1973) SV40 poly(A) signal SV40 poly(A) signal(2096-2217) SV40 ... SV40 poly(A) signal(2096-2217) SV40 ... SV40 poly(A) signal(2096-2217) f1 ori f1 ori(2224-2679) f1 or... f1 ori(2224-2679) AmpR promoter AmpR promoter(2706-2810) AmpR ... AmpR promoter(2706-2810) ...AmpR AmpR promoter(2706-2810) SV40 promoter SV40 promoter(2812-3169) SV40 ... SV40 promoter(2812-3169) SV40 ori SV40 ori(3020-3155) SV40 ... SV40 ori(3020-3155) ...SV40 SV40 ori(3020-3155) NeoR/KanR NeoR/KanR(3204-3998) NeoR/... NeoR/KanR(3204-3998) HSV TK poly(A) signal HSV TK poly(A) signal(4230-4277) HSV T... HSV TK poly(A) signal(4230-4277) ...HSV T HSV TK poly(A) signal(4230-4277) ori ori(4606-5194) ori ori(4606-5194) AmpR promoter AmpR promoter(2708-2812) AmpR ... AmpR promoter(2708-2812) ...AmpR AmpR promoter(2708-2812) SV40 ori SV40 ori(3022-3157) SV40 ... SV40 ori(3022-3157) ...SV40 SV40 ori(3022-3157) CMV promoter CMV promoter(367-570) CMV p... CMV promoter(367-570) CMV p... CMV promoter(367-570) SV40 promoter SV40 promoter(2814-3171) SV40 ... SV40 promoter(2814-3171) IRES2 IRES2(669-1255) IRES2 IRES2(669-1255) ori ori(4608-5196) ori ori(4608-5196) EGFP EGFP(1256-1975) EGFP EGFP(1256-1975) NeoR/KanR NeoR/KanR(3206-4000) NeoR/... NeoR/KanR(3206-4000)