3' LTR 3' LTR(1-634) 3' LT... 3' LTR(1-634) HIV-1 ψ HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) CMV enhancer CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV promoter CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) mCherry mCherry(2807-3514) mCher... mCherry(2807-3514) MCS MCS(3515-3580) MCS MCS(3515-3580) MCS MCS(3515-3580) PGK promoter PGK promoter(3613-4112) PGK p... PGK promoter(3613-4112) PuroR PuroR(4133-4732) PuroR PuroR(4133-4732) WPRE WPRE(4746-5334) WPRE WPRE(4746-5334) Factor Xa site Factor Xa site(5217-5228) Facto... Factor Xa site(5217-5228) ...Facto Factor Xa site(5217-5228) 3' LTR 3' LTR(5541-6174) 3' LT... 3' LTR(5541-6174) M13 rev M13 rev(6303-6319) M13 r... M13 rev(6303-6319) ...M13 r M13 rev(6303-6319) lac operator lac operator(6327-6343) lac o... lac operator(6327-6343) ...lac o lac operator(6327-6343) lac promoter lac promoter(6351-6381) lac p... lac promoter(6351-6381) ...lac p lac promoter(6351-6381) ori ori(6705-7293) ori ori(6705-7293) AmpR AmpR(7464-8324) AmpR AmpR(7464-8324) AmpR promoter AmpR promoter(8325-8429) AmpR ... AmpR promoter(8325-8429) ...AmpR AmpR promoter(8325-8429) SV40 poly(A) signal SV40 poly(A) signal(8477-8611) SV40 ... SV40 poly(A) signal(8477-8611) ...SV40 SV40 poly(A) signal(8477-8611) Factor Xa site Factor Xa site(5219-5230) Facto... Factor Xa site(5219-5230) ...Facto Factor Xa site(5219-5230) M13 rev M13 rev(6305-6321) M13 r... M13 rev(6305-6321) ...M13 r M13 rev(6305-6321) lac operator lac operator(6329-6345) lac o... lac operator(6329-6345) ...lac o lac operator(6329-6345) CAP binding site CAP binding site(6398-6419) CAP b... CAP binding site(6398-6419) ...CAP b CAP binding site(6398-6419) lac promoter lac promoter(6353-6383) lac p... lac promoter(6353-6383) ...lac p lac promoter(6353-6383) MCS MCS(3517-3582) MCS MCS(3517-3582) MCS MCS(3517-3582) AmpR promoter AmpR promoter(8327-8431) AmpR ... AmpR promoter(8327-8431) ...AmpR AmpR promoter(8327-8431) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) SV40 poly(A) signal SV40 poly(A) signal(8479-8613) SV40 ... SV40 poly(A) signal(8479-8613) ...SV40 SV40 poly(A) signal(8479-8613) CMV promoter CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) RRE RRE(1305-1538) RRE RRE(1305-1538) PGK promoter PGK promoter(3615-4114) PGK p... PGK promoter(3615-4114) PGK p... PGK promoter(3615-4114) WPRE WPRE(4748-5336) WPRE WPRE(4748-5336)