3' LTR 3' LTR(1-634) 3' LT... 3' LTR(1-634) HIV-1 ψ HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) CMV enhancer CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV promoter CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) MCS MCS(2793-2873) MCS MCS(2793-2873) MCS MCS(2793-2873) AcGFP1 AcGFP1(2875-3594) AcGFP... AcGFP1(2875-3594) PGK promoter PGK promoter(3622-4121) PGK p... PGK promoter(3622-4121) PuroR PuroR(4142-4741) PuroR PuroR(4142-4741) WPRE WPRE(4755-5343) WPRE WPRE(4755-5343) Factor Xa site Factor Xa site(5226-5237) Facto... Factor Xa site(5226-5237) ...Facto Factor Xa site(5226-5237) 3' LTR 3' LTR(5550-6183) 3' LT... 3' LTR(5550-6183) M13 rev M13 rev(6312-6328) M13 r... M13 rev(6312-6328) ...M13 r M13 rev(6312-6328) lac operator lac operator(6336-6352) lac o... lac operator(6336-6352) ...lac o lac operator(6336-6352) lac promoter lac promoter(6360-6390) lac p... lac promoter(6360-6390) ...lac p lac promoter(6360-6390) ori ori(6714-7302) ori ori(6714-7302) AmpR AmpR(7473-8333) AmpR AmpR(7473-8333) AmpR promoter AmpR promoter(8334-8438) AmpR ... AmpR promoter(8334-8438) ...AmpR AmpR promoter(8334-8438) SV40 poly(A) signal SV40 poly(A) signal(8486-8620) SV40 ... SV40 poly(A) signal(8486-8620) ...SV40 SV40 poly(A) signal(8486-8620) Factor Xa site Factor Xa site(5228-5239) Facto... Factor Xa site(5228-5239) ...Facto Factor Xa site(5228-5239) M13 rev M13 rev(6314-6330) M13 r... M13 rev(6314-6330) ...M13 r M13 rev(6314-6330) lac operator lac operator(6338-6354) lac o... lac operator(6338-6354) ...lac o lac operator(6338-6354) CAP binding site CAP binding site(6407-6428) CAP b... CAP binding site(6407-6428) ...CAP b CAP binding site(6407-6428) lac promoter lac promoter(6362-6392) lac p... lac promoter(6362-6392) ...lac p lac promoter(6362-6392) MCS MCS(2798-2875) MCS MCS(2798-2875) MCS MCS(2798-2875) MCS MCS(2795-2875) MCS MCS(2795-2875) MCS MCS(2795-2875) AmpR promoter AmpR promoter(8336-8440) AmpR ... AmpR promoter(8336-8440) ...AmpR AmpR promoter(8336-8440) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) SV40 poly(A) signal SV40 poly(A) signal(8488-8622) SV40 ... SV40 poly(A) signal(8488-8622) ...SV40 SV40 poly(A) signal(8488-8622) CMV promoter CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) RRE RRE(1305-1538) RRE RRE(1305-1538) PGK promoter PGK promoter(3624-4123) PGK p... PGK promoter(3624-4123) PGK p... PGK promoter(3624-4123) WPRE WPRE(4757-5345) WPRE WPRE(4757-5345) AcGFP1 AcGFP1(2877-3596) AcGFP... AcGFP1(2877-3596)