3' LTR 3' LTR(1-634) 3' LT... 3' LTR(1-634) HIV-1 ψ HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1... HIV-1 ψ(681-806) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) HIV-1... HIV-1 RRE(1303-1536) CMV enhancer CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV e... CMV enhancer(2199-2504) CMV promoter CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) CMV p... CMV promoter(2505-2708) IRES IRES(2843-3416) IRES IRES(2843-3416) PuroR PuroR(3449-4048) PuroR PuroR(3449-4048) WPRE WPRE(4062-4650) WPRE WPRE(4062-4650) Factor Xa site Factor Xa site(4533-4544) Facto... Factor Xa site(4533-4544) ...Facto Factor Xa site(4533-4544) 3' LTR 3' LTR(4857-5490) 3' LT... 3' LTR(4857-5490) M13 rev M13 rev(5618-5634) M13 r... M13 rev(5618-5634) ...M13 r M13 rev(5618-5634) lac operator lac operator(5642-5658) lac o... lac operator(5642-5658) ...lac o lac operator(5642-5658) lac promoter lac promoter(5666-5696) lac p... lac promoter(5666-5696) ...lac p lac promoter(5666-5696) ori ori(6020-6608) ori ori(6020-6608) AmpR AmpR(6779-7639) AmpR AmpR(6779-7639) AmpR promoter AmpR promoter(7640-7744) AmpR ... AmpR promoter(7640-7744) ...AmpR AmpR promoter(7640-7744) SV40 poly(A) signal SV40 poly(A) signal(7792-7926) SV40 ... SV40 poly(A) signal(7792-7926) ...SV40 SV40 poly(A) signal(7792-7926) Factor Xa site Factor Xa site(4535-4546) Facto... Factor Xa site(4535-4546) ...Facto Factor Xa site(4535-4546) M13 rev M13 rev(5620-5636) M13 r... M13 rev(5620-5636) ...M13 r M13 rev(5620-5636) lac operator lac operator(5644-5660) lac o... lac operator(5644-5660) ...lac o lac operator(5644-5660) CAP binding site CAP binding site(5713-5734) CAP b... CAP binding site(5713-5734) ...CAP b CAP binding site(5713-5734) lac promoter lac promoter(5668-5698) lac p... lac promoter(5668-5698) ...lac p lac promoter(5668-5698) AmpR promoter AmpR promoter(7642-7746) AmpR ... AmpR promoter(7642-7746) ...AmpR AmpR promoter(7642-7746) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) HIV-1... HIV-1 Ψ(683-808) SV40 poly(A) signal SV40 poly(A) signal(7794-7928) SV40 ... SV40 poly(A) signal(7794-7928) ...SV40 SV40 poly(A) signal(7794-7928) CMV promoter CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) CMV p... CMV promoter(2507-2710) RRE RRE(1305-1538) RRE RRE(1305-1538) IRES IRES(2956-3418) IRES IRES(2956-3418) WPRE WPRE(4064-4652) WPRE WPRE(4064-4652)