M13 fwd M13 fwd(379-395) M13 fwd M13 fwd(379-395) M13 fwd M13 fwd(379-395) MCS MCS(399-463) MCS MCS(399-463) M13 rev M13 rev(473-489) M13 rev M13 rev(473-489) M13 rev M13 rev(473-489) lac operator lac operator(497-513) lac operator lac operator(497-513) lac operator lac operator(497-513) lac promoter lac promoter(521-551) lac promoter lac promoter(521-551) lac promoter lac promoter(521-551) ori ori(875-1463) ori ori(875-1463) AmpR AmpR(1634-2494) AmpR AmpR(1634-2494) AmpR promoter AmpR promoter(2495-2599) AmpR promoter AmpR promoter(2495-2599) AmpR promoter AmpR promoter(2495-2599) M13 rev M13 rev(475-491) M13 rev M13 rev(475-491) M13 rev M13 rev(475-491) M13 fwd M13 fwd(381-397) M13 fwd M13 fwd(381-397) M13 fwd M13 fwd(381-397) lac operator lac operator(499-515) lac operator lac operator(499-515) lac operator lac operator(499-515) CAP binding site CAP binding site(568-589) CAP binding site CAP binding site(568-589) CAP binding site CAP binding site(568-589) lac promoter lac promoter(523-553) lac promoter lac promoter(523-553) lac promoter lac promoter(523-553) AmpR promoter AmpR promoter(2497-2601) AmpR promoter AmpR promoter(2497-2601) AmpR promoter AmpR promoter(2497-2601)