traJ traJ(145-516) traJ traJ(145-516) oriT oriT(549-658) oriT oriT(549-658) oriT oriT(549-658) TetR TetR(1339-1989) TetR TetR(1339-1989) NOS promoter NOS promoter(2602-2781) NOS p... NOS promoter(2602-2781) NOS p... NOS promoter(2602-2781) NOS promoter NOS promoter(2634-2817) NOS p... NOS promoter(2634-2817) NOS p... NOS promoter(2634-2817) NeoR NeoR(2838-3632) NeoR NeoR(2838-3632) NOS terminator NOS terminator(4022-4274) NOS t... NOS terminator(4022-4274) NOS t... NOS terminator(4022-4274) CAP binding site CAP binding site(4823-4844) CAP b... CAP binding site(4823-4844) CAP b... CAP binding site(4823-4844) lac promoter lac promoter(4859-4889) lac p... lac promoter(4859-4889) lac p... lac promoter(4859-4889) lac operator lac operator(4897-4913) lac o... lac operator(4897-4913) lac o... lac operator(4897-4913) CaMV 35S promoter CaMV 35S promoter(5461-5806) CaMV ... CaMV 35S promoter(5461-5806) CaMV ... CaMV 35S promoter(5461-5806) mCherry mCherry(5845-6555) mCher... mCherry(5845-6555) oriV oriV(12910-18) oriV oriV(12910-18) NOS terminator NOS terminator(6572-6824) NOS t... NOS terminator(6572-6824) NOS t... NOS terminator(6572-6824) LB T-DNA repeat LB T-DNA repeat(7467-7491) LB T-... LB T-DNA repeat(7467-7491) ...LB T- LB T-DNA repeat(7467-7491) trfA trfA(9087-10235) trfA trfA(9087-10235) KanR KanR(10534-11328) KanR KanR(10534-11328) IS1 IS1(11520-12287) IS1 IS1(11520-12287) M13 rev M13 rev(4923-4939) M13 r... M13 rev(4923-4939) M13 r... M13 rev(4923-4939) M13 fwd M13 fwd(6835-6851) M13 f... M13 fwd(6835-6851) ...M13 f M13 fwd(6835-6851) lac operator lac operator(4899-4915) lac o... lac operator(4899-4915) lac o... lac operator(4899-4915) CAP binding site CAP binding site(4825-4846) CAP b... CAP binding site(4825-4846) CAP b... CAP binding site(4825-4846) LB T-DNA repeat LB T-DNA repeat(7471-7493) LB T-... LB T-DNA repeat(7471-7493) ...LB T- LB T-DNA repeat(7471-7493) RB T-DNA repeat RB T-DNA repeat(2456-2480) RB T-... RB T-DNA repeat(2456-2480) RB T-... RB T-DNA repeat(2456-2480) LB T-DNA repeat LB T-DNA repeat(7469-7493) LB T-... LB T-DNA repeat(7469-7493) ...LB T- LB T-DNA repeat(7469-7493) lac promoter lac promoter(4861-4891) lac p... lac promoter(4861-4891) lac p... lac promoter(4861-4891) oriT oriT(551-660) oriT oriT(551-660) oriT oriT(551-660) NOS promoter NOS promoter(2604-2783) NOS p... NOS promoter(2604-2783) NOS p... NOS promoter(2604-2783) NOS promoter NOS promoter(2636-2819) NOS p... NOS promoter(2636-2819) NOS p... NOS promoter(2636-2819) NOS terminator NOS terminator(4024-4276) NOS t... NOS terminator(4024-4276) NOS t... NOS terminator(4024-4276) NOS terminator NOS terminator(6574-6826) NOS t... NOS terminator(6574-6826) NOS t... NOS terminator(6574-6826) traJ traJ(147-518) traJ traJ(147-518) traJ traJ(147-518) TetR TetR(1341-1991) TetR TetR(1341-1991) KanR KanR(10536-11330) KanR KanR(10536-11330) trfA trfA(9089-10237) trfA trfA(9089-10237)