T7 promoter T7 promoter(20-38) T7 pr... T7 promoter(20-38) T7 pr... T7 promoter(20-38) 6xHis 6xHis(112-129) 6xHis 6xHis(112-129) 6xHis 6xHis(112-129) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(133-165) T7 ta... T7 tag (gene 10 leader)(133-165) T7 ta... T7 tag (gene 10 leader)(133-165) Xpress? tag Xpress? tag(169-192) Xpres... Xpress? tag(169-192) Xpres... Xpress? tag(169-192) T7 terminator T7 terminator(312-359) T7 te... T7 terminator(312-359) T7 te... T7 terminator(312-359) f1 ori f1 ori(456-911) f1 or... f1 ori(456-911) AmpR promoter AmpR promoter(937-1041) AmpR ... AmpR promoter(937-1041) AmpR ... AmpR promoter(937-1041) AmpR AmpR(1042-1902) AmpR AmpR(1042-1902) ori ori(2073-2661) ori ori(2073-2661) T7 promoter T7 promoter(22-40) T7 pr... T7 promoter(22-40) T7 pr... T7 promoter(22-40) Xpress tag Xpress tag(171-194) Xpres... Xpress tag(171-194) Xpres... Xpress tag(171-194) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(135-167) T7 ta... T7 tag (gene 10 leader)(135-167) T7 ta... T7 tag (gene 10 leader)(135-167) T7 terminator T7 terminator(314-361) T7 te... T7 terminator(314-361) T7 te... T7 terminator(314-361) AmpR promoter AmpR promoter(939-1043) AmpR ... AmpR promoter(939-1043) AmpR ... AmpR promoter(939-1043)