AmpR promoter AmpR promoter(104-208) AmpR ... AmpR promoter(104-208) AmpR ... AmpR promoter(104-208) AmpR AmpR(209-1069) AmpR AmpR(209-1069) ori ori(1240-1828) ori ori(1240-1828) rop rop(2258-2449) rop rop(2258-2449) lacI lacI(3761-4843) lacI lacI(3761-4843) lacI promoter lacI promoter(4844-4921) lacI ... lacI promoter(4844-4921) ...lacI lacI promoter(4844-4921) T7 promoter T7 promoter(5230-5248) T7 pr... T7 promoter(5230-5248) ...T7 pr T7 promoter(5230-5248) lac operator lac operator(5249-5273) lac o... lac operator(5249-5273) ...lac o lac operator(5249-5273) 6xHis 6xHis(5329-5346) 6xHis 6xHis(5329-5346) 6xHis 6xHis(5329-5346) thrombin site thrombin site(5356-5373) throm... thrombin site(5356-5373) ...throm thrombin site(5356-5373) T7 terminator T7 terminator(5449-5496) T7 te... T7 terminator(5449-5496) ...T7 te T7 terminator(5449-5496) tet promoter tet promoter(5671-5699) tet p... tet promoter(5671-5699) ...tet p tet promoter(5671-5699) lac operator lac operator(5255-5271) lac o... lac operator(5255-5271) ...lac o lac operator(5255-5271) thrombin site thrombin site(5358-5375) throm... thrombin site(5358-5375) ...throm thrombin site(5358-5375) T7 promoter T7 promoter(5232-5250) T7 pr... T7 promoter(5232-5250) ...T7 pr T7 promoter(5232-5250) lac operator lac operator(5251-5275) lac o... lac operator(5251-5275) ...lac o lac operator(5251-5275) tet promoter tet promoter(5673-5701) tet p... tet promoter(5673-5701) ...tet p tet promoter(5673-5701) T7 terminator T7 terminator(5451-5498) T7 te... T7 terminator(5451-5498) ...T7 te T7 terminator(5451-5498) lacI promoter lacI promoter(4846-4923) lacI ... lacI promoter(4846-4923) ...lacI lacI promoter(4846-4923) AmpR promoter AmpR promoter(106-210) AmpR ... AmpR promoter(106-210) AmpR ... AmpR promoter(106-210) rop rop(2260-2451) rop rop(2260-2451) ori ori(1242-1830) ori ori(1242-1830) AmpR AmpR(211-1071) AmpR AmpR(211-1071)